论文部分内容阅读
目的 以超排卵下小鼠子宫内膜种植窗期整合素 β3的表达为指标 ,比较不同超排卵方案对小鼠子宫内膜容受性的影响。方法 模拟人类超排卵长周期 ,对小鼠进行超促排卵。根据超排卵方案的不同 ,按随机表顺序将 4 0只小鼠分为 4组 ,分别为促性腺激素释放激素拮抗剂 (GnRHant)组、促性腺激素释放激素激动剂 (GnRHa)组、孕马血清促性腺激素 (PMSG)组及对照组 ,每组 10只小鼠。用免疫组织化学和逆转录 聚合酶链反应 (RT PCR)方法分别检测小鼠子宫内膜种植窗期整合素β3蛋白和mRNA的表达。结果 免疫组织化学结果显示 ,与对照组相比GnRHa组小鼠整合素 β3蛋白的表达明显下降 (P =0 0 2 3) ,GnRHant组和PMSG组小鼠整合素 β3蛋白的表达明显降低 (P =0 0 0 1) ;与PMSG组小鼠相比 ,GnRHa组小鼠整合素 β3的表达明显增高 (P =0 0 0 1) ,而GnRHant组整合素 β3的表达差异无显著意义 (P =0 76 8) ;RT PCR结果与免疫组化结果相符。 结论与小鼠自然周期相比 ,3种超排卵方案均使小鼠子宫内膜种植窗期整合素 β3的表达明显下降 ,可能降低了子宫内膜容受性 ;与单一PMSG超排卵方案相比 ,GnRHa辅助超排卵方案能明显改善小鼠子宫内膜的容受性 ;而GnRHant辅助超排卵方案则对小鼠子宫内膜容受性的改善作用不明显。
Objective To investigate the effect of different ovarian hyperstimulation protocols on the endometrial receptivity in mouse endometrium underglauding. Methods To simulate the long-term human superovulation, ovarian hyperstimulation in mice. According to the different ovarian superovulation scheme, 40 mice were randomly divided into 4 groups according to random table sequence: GnRHant group, GnRHa group, Serum gonadotropin (PMSG) group and control group, each group of 10 mice. Immunohistochemistry and reverse transcription polymerase chain reaction (RT PCR) were used to detect the expression of integrin β3 protein and mRNA at the implantation window of mouse endometrium respectively. Results The results of immunohistochemistry showed that the expression of integrin β3 protein in GnRHa group was significantly lower than that in control group (P = 0.0023), while the expression of integrin β3 protein in GnRHant group and PMSG group was significantly decreased (P = 0 0 0 1). Compared with PMSG mice, the expression of integrin β3 was significantly increased in GnRHa mice (P = 0.001), but there was no significant difference in integrin β3 expression in GnRHant mice (P = 0 76 8); RT PCR results consistent with the results of immunohistochemistry. CONCLUSIONS: Compared with the natural cycle of mice, the three superovulation schemes significantly decreased the expression of integrin β3 at the implantation window of mice endometrium, which may reduce the endometrial receptivity. Compared with the single PMSG superovulation scheme , GnRHa assisted superovulation can significantly improve the endometrial receptivity of mice; while the GnRHant-assisted superovulation has no effect on the improvement of endometrial receptivity in mice.