论文部分内容阅读
目的在微重力环境下内分泌激素有一过性的变化，但是不能解释主要在承重骨发生矿盐丢失的现象。近年来的研究表明，骨矿盐丢失主要与骨质中的局部因子有关。去负荷骨不同部位的局部因子与骨丢失的关系目前还不十分清楚。本研究旨在探讨大鼠股骨去负荷后局部因子和矿盐变化的关系。方法利用悬吊大鼠模型来模拟航天骨去负荷的变化，观察了股骨４个不同部位一氧化氮（ＮＯ）、骨钙素（ＢＧＰ）、表皮生长因子（ＥＧＦ）的含量及矿盐含量。６周龄ＳＤ大鼠随机分为自由活动组和悬吊组，每组９只，实验期２１天。用灰分法检测骨矿盐含量，用放免法和Ｇｒｉｅｓｓ法分别检测骨样品匀浆液中ＥＧＦ、ＢＧＰ和ＮＯ的含量。结果与自由对照组相比，悬吊组大鼠股骨颈和骨干远１／３段的矿盐含量显著下降（Ｐ＜０．０５），ＮＯ含量显著升高（Ｐ＜０．０５）；股骨干中１／３段的ＢＧＰ和ＥＧＦ含量显著升高（Ｐ＜０．０５）；股骨干近１／３段的ＥＧＦ含量也显著升高（Ｐ＜０．０５）。结论去负荷大鼠股骨颈和股骨干远１／３段的ＮＯ含量升高，是造成该部位矿盐含量下降的重要原因。股骨干近、中１／３段的ＥＧＦ或ＢＧＰ含量升高是该部位矿盐无明显丢失的重要原因。
OBJECTIVE: Endocrine hormones undergo transient changes in the microgravity environment, but can not explain the loss of mineral salts mainly in the bearing bones. In recent years, studies have shown that the loss of bone mineral mainly with the local factors in the bone. The relationship between local factors and bone loss in different parts of the bone is not yet clear. The purpose of this study was to investigate the relationship between local factors and mineral salts after loading of femur in rats. Methods Suspension rat model was used to simulate the change of astroglias detrusor load. The contents of NO, BGP, EGF and mineral salts in 4 different parts of femur were observed. Six-week-old SD rats were randomly divided into free-moving group and suspension group, with 9 in each group and 21 days in experimental group. Bone mineral content was detected by ash method. The content of EGF, BGP and NO in homogenate of bone samples were detected by radioimmunoassay and Griess method respectively. Results Compared with the control group, the content of mineral salt in the femoral neck and the distal part of the backbone was significantly decreased (P <0.05) and the content of NO was significantly increased in the suspension group (P <0.05) The content of BGP and EGF in the middle 1/3 of the trunk was significantly increased (P <0.05), and the content of EGF in the proximal 1/3 of the femur was also significantly increased (P <0.05). Conclusion The NO content in the far 1/3 of the femoral neck and femoral shaft in the unloaded rats is an important reason for the decrease of mineral salt content in this part. Near the femoral shaft, the increase of the content of EGF or BGP in the middle 1/3 section is an important reason for no significant loss of mineral salt in this site.