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目的:用HPLC-ELSD法测定脑得生片中三七皂苷R1和人参皂苷Rg1、Rb1含量。方法:采用Hypersil ODS-C18色谱柱(4.6mm×250mm,5μm),以乙腈-水为流动相,梯度洗脱(0～20min,乙腈20%→40%;20～26min,乙腈40%→20%),流速1.0mL·min-1,柱温25℃,漂移管温度40℃,载气压力3.5kPa。结果:三七皂苷R1和人参皂苷Rg1、Rb1进样量分别在0.150～3.00μg(r=0.9991)、0.753～15.1μg(r=0.9996)和0.755～15.1μg(r=0.9995)范围内呈良好的线性关系;平均加样回收率(n=6)分别为98.3%(RSD=1.0%),96.7%(RSD=1.5%),98.6%(RSD=0.9%)。结论:该方法快速、简便、准确,可用于脑得生片的质量控制。
Objective: To determine the content of notoginsenoside R1 and ginsenoside Rg1 and Rb1 in Naodesheng Tablets by HPLC-ELSD method. Method: Hypersil ODS-C18 column (4.6mm×250mm, 5μm) was used. The mobile phase was acetonitrile-water. Gradient elution was used (0～20min, acetonitrile 20%→40%; 20～26min, acetonitrile 40%→20. %), flow rate 1.0 mL·min-1, column temperature 25°C, drift tube temperature 40°C, carrier gas pressure 3.5 kPa. RESULTS: The amount of notoginsenoside R1 and ginsenoside Rg1 and Rb1 were good in the range of 0.150-3.00 μg (r=0.9991), 0.753-15.1 μg (r=0.9996) and 0.755-15.1 μg (r=0.9995), respectively. The linear relationship; average recovery (n=6) was 98.3% (RSD=1.0%), 96.7% (RSD=1.5%), and 98.6% (RSD=0.9%), respectively. Conclusion: This method is rapid, simple, accurate and can be used for the quality control of Naodesheng tablets.