论文部分内容阅读
目的:建立反相高效液相色谱法测定普伐他汀在大鼠血浆中的含量。方法:空白血浆加普伐他汀和内标,用Bond E-lut C18小柱进行固相提取,然后用色谱方法分析。采用Puroshper RP-C18反相色谱柱（5μm,250mm×4mm）,Lichrospher 100RP-18预柱（5μm,4mm×4mm）,流动相为0.035mmol·L-1磷酸二氢钠缓冲液（用磷酸调pH3.0）-乙腈（13:7）,流速1mL·min-1,检测波长239nm,柱温40℃,进样量100μL。结果:普伐他汀在血中的线性范围为10～400ng·mL-1,r=0.9997;最低检测限用标准液试验达2ng·mL（S/N=3）,用加标样血浆试验,方法定量限为10ng·mL-1血,RSD<20％;不同浓度固相提取回收率平均为71.48％;日内差平均为13％,日间差平均为19％。用上述色谱方法测定了普伐他汀在大鼠血浆中（Lewis大鼠,灌胃20mg·kg-1）的含量,并绘制药时曲线。结论:方法实用有效,可用于药代动力学研究。
Objective: To establish an RP-HPLC method for the determination of pravastatin in rat plasma. Methods: Blank plasma plus pravastatin and internal standard was extracted with solid phase using Bond E-lut C18 cartridge and analyzed by chromatography. A Purified RP-C18 reversed-phase column (5 μm, 250 mm × 4 mm), a Lichrospher 100 RP-18 precolumn (5 μm, 4 mm × 4 mm) was used. The mobile phase was 0.035 mmol·L -1 sodium dihydrogen phosphate buffer pH3.0) -acetonitrile (13: 7), flow rate 1mL · min-1, detection wavelength 239nm, column temperature 40 ℃, injection volume 100μL. Results: The linear range of pravastatin in blood was 10 ~ 400 ng · mL-1, r = 0.9997. The lowest detection limit was 2 ng · mL (S / N = 3) The limit of quantification was 10 ng · mL-1 and the RSD was less than 20%. The average recoveries were 71.48% at different concentrations. The average difference between days was 13% and the average difference between days was 19%. The content of pravastatin in rat plasma (Lewis rats, intragastric administration of 20 mg · kg-1) was determined by the above chromatographic method, and the time curve of the drug was drawn. Conclusion: The method is practical and effective and can be used in pharmacokinetic studies.