【摘 要】
:
Background:The hyper-prolificacy Meishan gilts achieved a superior endometrial gland development (EGD) than white crossbred gilts during the ovary-independent period (before 60 d of age). Then, the EGD continues under the management of ovary-derived stero
【机 构】
:
State Key Laboratory of Agricultural Microbiology,College of Animal Sciences and Technology,Huazhong
论文部分内容阅读
Background:The hyper-prolificacy Meishan gilts achieved a superior endometrial gland development (EGD) than white crossbred gilts during the ovary-independent period (before 60 d of age). Then, the EGD continues under the management of ovary-derived steroid hormones that regulated by gut microbiota (after 60 d of age). However, whether Meishan gilts\' superiority in EGD lasting to the ovary-dependent period (after 60 d of age) and the role of gut microbiota in this period both remain unclear. Methods:Meishan gilts and Landrace x Yorkshire (LxY) gilts were raised under the same housing and feeding conditions until sexual maturity and then we compared their EGD and gut microbiota. Meanwhile, we transplanted fecal microbiota from Meishan gilts to L×Y gilts to explore the role of gut microbiota in EGD. We sampled plasma every 3 weeks and collected the uterus, ovary, liver, and rectal feces after the sacrifice. We then determined the hormone concentrations and expressions of the EGD-related genes. We also profiled the gut microbiota using 16S rDNA sequencing and metabolites of plasma and liver tissue using untargeted metabolomics. Finally, the correlation analysis and significant test was conducted between FMT-shifted gut microbes and EGD-related indices. Results:Meishan gilts have larger endometrial gland area (P<0.001), longer uterine horn length (P<0.01) but lighter uterine horn weight (P<0.05), a distinctive gut microbiota compared with L×Y gilts. Fecal microbiota transplantation (FMT) increased endometrial gland area (P<0.01). FMT markedly shifted the metabolite profiles of both liver and plasma, and these differential metabolites enriched in steroid hormone biosynthesis pathway. FMT increased estradiol and insulin-like growth factor 1 but decreased progesterone dynamically. FMT also increased the expression of the EGD-related genes estrogen receptor 1 gene, epithelial cadherin, and forkhead box protein A2. There is a significant correlation between FMT-shifted gut microbes and EGD-related indices. Conclusion:Sexually matured Meishan gilts achieved a superior EGD than LxY gilts. Meanwhile, gut microbiota contribute to the EGD potentially via regulating of steroid hormones during the ovary-dependent period.
其他文献
浙江省温岭市农技人员在20 世纪末探索了一套设施西瓜长季节栽培技术,全年只种植1 茬西瓜,每667 m2 产值0.8 万~1.0 万元,纯收益4 000元,该技术不仅被推广到全国各地,甚至推广至越南、老挝等国家.
航研998是由平韭4号太空诱变材料经5代连续自交育成的韭菜新品种.株型高大直立,叶片长宽条形、深绿色,叶长38 cm左右,叶宽0.8 cm左右;生长势强,冬季不休眠,田间对灰霉病的抗性强于对照791,VC含量220 mg·kg-1;年收割6刀左右,平均单株质量9.5 g,每667 m2产量13000 kg左右,适宜河南省及其周边地区种植.
夏秋美味是以雄性不育系EC-2为母本,以自交系312为父本配制而成的中晚熟菜薹一代杂种.株型半直立,生长势强,基叶阔卵圆形、嫩绿色;主薹粗壮、绿色,覆轻微蜡粉,薹长29 cm左右,单薹质量82 g左右,薹质细腻甜糯,商品性好;抽薹整齐,可一次性采收,也可主侧薹兼收,VC含量577.0 mg·kg-1,可溶性糖含量2.22%,粗纤维含量0.6%,每667 m2产量2300 kg左右,适宜苏南及其周边地区种植.
Background:Intestinal disorder is an important factor contributing to growth lag and high rates of morbidity and mortality of piglets with intrauterine growth retardation (IUGR). Resveratrol (RSV) and its derivative pterostilbene (PT) are natural stilbene
为探索肥药减量技术集成应用的效果,通过试验比较了不同生产技术模式对病虫害的综合防控效果、田间昆虫群落特征、果实营养品质和商品性的影响,采用环境经济学方法评价了肥药减量技术应用于生产的实际效果及综合效益.结果表明,肥药减量技术模式对保护地栽培南瓜主要病虫害的防控效果显著优于其他处理区;可在化肥使用减量66.7%、化学农药使用减量46.3%的情况下,减轻病虫危害损失,主要害虫优势度指数降低,天敌数量和作用增加,昆虫群落丰富度、多样性指数和均匀度指数上升,生态系统的服务功能得到提高;肥药减量区单果质量、果实横径
马 铃 薯 瓢 虫〔Henosepilachna vigintioctomaculata(Motschulsky)〕别名大二十八星瓢虫,属鞘翅目瓢虫科,与茄二十八星瓢虫?〔Henosepilachna vigintioctopunctata(Fabricius)〕均为植食性瓢虫,二者常混合发生,不仅形态习性相似,取食寄主、为害症状也较为相似,都是以成虫和幼虫为害茄科、葫芦科、豆科、十字花科、菊科、百合科等植物,其中以马铃薯、茄子、辣椒等茄科作物或龙葵、蔓陀萝等杂草受害最为严重(刘国英,2013;司升云 等
嫩南瓜是以采收、鲜食嫩瓜为主的新一代南瓜类型,因地头出货价较为稳定而受到农户欢迎.近年来,广东粤北及惠州地区的嫩南瓜种植面积逐年增加,仅一串铃系列嫩南瓜品种(湖南省衡阳市蔬菜研究所选育)在广东地区就已经累计推广1? 067 hm2(1.6万亩)以上,成为珠三角地区重要的瓜菜种类之一.由于地理、气候、人文等差异,惠州地区嫩南瓜一般采用“人”字架栽培模式,粤北地区则以平棚架栽培模式为主.但以上栽培模式存在棚架材料笨拙、肥水供应方式原始、品种选择途径狭窄等问题.为此,笔者团队结合生产实际设计了一种轻便耐用的镶接
Background:Sows are frequently subjected to various stresses during late gestation and lactation, which trigger inflammatory response and metabolic disorders. Dietary fiber can influence animal health by modulating gut microbiota and their by-products, wi
利用不同温度对红托竹荪(Dictyophora rubrovalvata)母种进行培养,通过对菌丝密度、均匀度、长满平板时间、菌丝生长速度等指标进行测定,以便筛选出红托竹荪菌丝生长的最适温度.结果表明最适合红托竹荪母种培养的温度为25℃,在低温条件下菌种可微弱生长,在恢复到合适温度时会正常生长.
江苏省东台市是全国闻名的早春设施西瓜生产基地,常年种植面积近2万hm2(30万亩),总产量达100万t,效益30亿元.西瓜种植属于劳动密集型产业,近年来随着劳动力短缺、人工成本增加、农时用工紧张等问题的出现,影响了东台西瓜栽培规模的进一步扩大.2019年东台市农业机械化技术推广服务站开展了“东台西瓜生产主要环节机械化装备与技术推广应用”项目研究,在耕翻撒肥、整地起垄、建棚覆膜、播种育苗、覆膜移栽、环境调控、灌溉追肥、病虫害防控、机械运输、尾菜处理等关键环节采用适宜机械,使西瓜生产机械化水平达到80%以上,