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目的:对比分析微柱凝胶卡式法(MGT)和试管抗人球蛋白法(TAT)在检测新生儿ABO溶血三项试验中的应用。方法:对疑为新生儿溶血病的血标本123份采用MGT法与TAT法进行新生儿ABO溶血三项试验:即直抗试验、游离试验和释放试验,并将数据进行统计学处理。结果:MGT法与TAT法两法直抗试验检测的阳性率分别为26.8%和21.1%,游离试验阳性率分别为54.5%和46.3%,释放试验阳性率分别为65.9%和53.7%。两法阳性率比较差异无统计学意义(P>0.05);而两法凝集强度的比较差异有统计学意义(P=0.000),MGT法强于TAT法。结论:新生儿ABO溶血三项试验检测中,MGT法有快速、简便、敏感性和重复性强、结果判定直观并易保存等特点,优于TAT法。
OBJECTIVE: To compare and analyze the application of microtitre gel card method (MGT) and in vitro anti-human globulin (TAT) assay in the three tests of neonatal ABO hemolysis. Methods: Three hundred and three blood samples suspected to be hemolytic disease of newborn were tested by MGT method and TAT method in three ABO hemolytic tests: direct resistance test, free test and release test, and the data were statistically processed. Results: The positive rates of MGT and TAT were 26.8% and 21.1% respectively. The positive rates of free test were 54.5% and 46.3% respectively. The positive rates of release test were 65.9% and 53.7% respectively. There was no significant difference between the two methods (P> 0.05). The agglutination intensity of the two methods was significantly different (P = 0.000). The MGT method was stronger than the TAT method. Conclusion: Among the three tests of neonatal ABO hemolysis, the MGT method is quick, simple, sensitive and repeatability, the results are intuitive and easy to save, which is better than the TAT method.