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目的 :探讨人内皮细胞中一种新的可溶型黏附分子sCD2 2 6的表达调变规律。方法 :用ELISA方法 ,检测不同浓度的LPS刺激人脐静脉内皮细胞后不同时间点培养上清中sCD2 2 6含量的变化 ;用Greiss法检测上述细胞培养上清中NO含量的变化 ,并分析两者的线性相关性。加入抗TNF α中和抗体和iNOS抑制剂后 ,观察其对LPS诱导sCD2 2 6生成的影响。结果 :①随着LPS刺激剂量的增加和刺激时间的延长 ,HUVEC培养上清中sCD2 2 6的含量明显增加 ,10 0mg/LLPS刺激 3d后sCD2 2 6的含量为 188.5 μg/L。②LPS刺激剂量为10 0mg/L时 ,HUVEC培养上清中sCD2 2 6和NO的含量呈显著的正相关。③抗TNF α中和抗体和iNOS抑制剂可显著抑制LPS活化的HUVEC产生sCD2 2 6。结论 :LPS可诱导HUVEC产生sCD2 2 6分子 ,且sCD2 2 6的生成与NO和TNF α的产生密切相关
Objective: To investigate the regulation of expression of a novel soluble sCD226 in human endothelial cells. Methods: The changes of sCD2 2 6 content in supernatant of culture supernatant of human umbilical vein endothelial cells stimulated with different concentrations of LPS were detected by ELISA. The changes of NO content in supernatants were detected by Greiss method. The linear correlation of people. After addition of anti-TNF α neutralizing antibody and iNOS inhibitor, the effect of LPS on sCD22 6 production was observed. Results: ① With the increase of LPS stimulation dose and the prolongation of stimulation time, the content of sCD2 2 6 in supernatant of HUVEC increased obviously, and the content of sCD2 2 6 was 188.5 μg / L after 3 days of stimulation with 100 mg / L LLPS. ② When LPS stimulation dose was 10 0 mg / L, the content of sCD2 2 6 and NO in culture supernatant of HUVEC showed a significant positive correlation. ③ Anti-TNF α neutralizing antibody and iNOS inhibitor can significantly inhibit the production of sCD22 6 in LPS-activated HUVECs. CONCLUSION: LPS can induce sCD26 6 production in HUVEC and the generation of sCD2 2 6 is closely related to the production of NO and TNFα