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目的建立同时测定三七提取液中三七皂苷R2(S)、七叶胆苷XVII及人参皂苷F2含量的高效液相色谱法。方法色谱柱为Waters Acquity UPLC CSH-C18(50 mm×2.1 mm,1.7μm),流动相为0.01%甲酸-水(A)和0.01%甲酸-乙腈(B),梯度洗脱,检测波长为203 nm,进样量为5μL,流速为0.35 m L·min?1,柱温为40℃。结果在43 min内可完成三七皂苷R2(S)、七叶胆苷XVII和人参皂苷F2的分离测定。3种皂苷峰面积和浓度线性关系良好(r2>0.999 8);日内和日间精密度RSD<3.4%;回收率为98.4%~102.1%。结论该方法简便、准确,重复性好,可用于三七提取液中皂苷成分的测定。
OBJECTIVE To establish a HPLC method for the simultaneous determination of notoginsenoside R2 (S), aescine cholecalciferol XVII and ginsenoside F2 in Panax notoginseng extract. Methods The mobile phase consisted of Waters Acquity UPLC CSH-C18 (50 mm × 2.1 mm, 1.7 μm) with a mobile phase of 0.01% formic acid-water (A) and 0.01% formic acid- The injection volume was 5μL, the flow rate was 0.35 m L · min -1, and the column temperature was 40 ℃. Results The isolation and determination of notoginsenoside R2 (S), aesculin XVII and ginsenoside F2 were completed in 43 min. The linear relationship between the three saponins’ peak area and concentration was good (r2> 0.999 8). The intra-day and inter-day RSDs were less than 3.4% and the recoveries were 98.4% -102.1%. Conclusion The method is simple, accurate and reproducible. It can be used for the determination of saponin in Panax notoginseng extract.