论文部分内容阅读
利用大鼠胚胎中脑神经元细胞进行微团培养,研究了锰的细胞毒性及其对神经元分化的影响。细胞毒性试验提示锰的半数存活抑制浓度（ICV50）为18.0μmol／L。染毒组的细胞集落形成率降低，细胞体积小，细胞间神经纤维减少等形态学变化，其中前者呈剂量-效应关系。表明锰可抑制胚胎神经元细胞的分化，半数分化抑制浓度（ICD50）为10.0μmol／L，半数存活抑制浓度与半数分化抑制浓度的比值（V／D）均大于1.8。表明抑制神经元细胞分化可能是锰致发育危害的重要作用机制。
The rat embryonic midbrain neurons were cultured in micelles to study the cytotoxicity of manganese and its effect on neuronal differentiation. Cytotoxicity assays suggested that the half-life inhibitory concentration of manganese (ICV50) was 18.0 μmol / L. The rate of colony formation, the small cell size and the decrease of intercellular nerve fibers in the exposed group were observed. The former showed dose-effect relationship. The results showed that manganese could inhibit the differentiation of embryonic neurons. ICD50 was 10.0μmol / L, and the ratio of half survival inhibition to half inhibition was greater than 1.8. Indicating that inhibition of neuronal cell differentiation may be an important mechanism of action of manganese-induced development.