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目的:观察硫磺熏蒸山药对大鼠肝组织内抗氧化能力,Ca2+Mg2+-ATPase,Na+K+-ATPase活性的变化。方法:将实验动物随机分为4组,其中3组分别给予未熏蒸山药、市售品山药、硫磺熏蒸山药水提浓缩液(5 g.mL-1),掺入基础饲料,50g.kg-1 BW;正常对照组给予基础饲料,测定14周后大鼠肝组织中MDA含量、总超氧化物歧化酶(SOD)活力、谷胱甘肽过氧化物酶(GSH-Px)活力,Ca2+Mg2+-ATPase,Na+K+-ATPase活性;同时观察肝组织结构的病理变化。结果:经口给予受试物14周,两性动物未薰山药组、市售山药组肝组织中GSH-PX活力与对照组比较均显著增加,均有显著性差异(P<0.05或P<0.01);市售山药组雌性大鼠肝组织内Na+K+-ATPase活性比对照组高,有显著性差异(P<0.05)。结论:长期服用经大量硫磺薰制后的山药可能影响肝组织的氧化应激系统和Na+K+-ATPase过程。
OBJECTIVE: To observe the changes of anti-oxidation ability, Ca2+Mg2+-ATPase and Na+K+-ATPase activity in liver tissue of rats exposed to sulfur fumigation. METHODS: The experimental animals were randomly divided into 4 groups, in which 3 groups were given non-fumigated yam, commercial yam, sulfur fumigation yam extract concentrate (5 g.mL-1), and basal feed, 50 g.kg- 1 BW; Normal control group was given basal diet, and MDA content, total superoxide dismutase (SOD) activity, and glutathione peroxidase (GSH-Px) activity in rat liver tissue after 14 weeks were measured. Mg2+-ATPase, Na+K+-ATPase activity; At the same time observe the pathological changes of liver tissue structure. RESULTS: After oral administration of the test substance for 14 weeks, the GSH-PX activity in the liver tissue of untreated yam group and commercial yam group was significantly increased compared with the control group, with significant difference (P<0.05 or P<0.01). The activity of Na+K+-ATPase in liver tissue of female rats in the commercial yam group was higher than that in the control group, with significant difference (P<0.05). Conclusion: Long-term administration of yam with large amounts of sulfur may affect the oxidative stress system and Na+K+-ATPase process in liver tissue.