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目的分析乙型肝炎病毒大蛋白(L HBS)与HBV-DNA的相关性,探讨L HBS在乙型肝炎临床检测中的意义。方法以ELISA法检测样品L HBS和定量PCR法检测HBV-DNA。结果218例HBsAg阳性和HBeAg阳性的血清样本中,L HBS阳性率为95.41%,HBV-DNA的阳性率为92.66%,两者阳性率差异统计学意义(χ2=81.36,P<0.001);120例HBsAg阳性、HBeAg阴性的血清样本中,L HBS阳性率为65.00%,HBV-DNA的阳性率为68.33%,两者阳性率差异统计学意义(χ2=47.21,P<0.001)。L HBS水平与HBV-DNA拷贝数呈直线正相关性(r=0.965)。结论L HBS和HBV-DNA的检测敏感新有差异,但相关性较强。L HBS可检测感染者体内病毒复制程度,特别是对HBeAg阴性的患者。
Objective To analyze the correlation between hepatitis B virus large protein (L HBS) and HBV-DNA and to explore the significance of L HBS in the clinical detection of hepatitis B. Methods Detection of HBV-DNA by L-HBS ELISA and quantitative PCR. Results The positive rate of L HBS in 218 HBsAg-positive and HBeAg-positive sera was 95.41% and the positive rate of HBV-DNA was 92.66%, the difference was statistically significant (χ2 = 81.36, P <0.001); In HBsAg-positive and HBeAg-negative serum samples, the positive rate of L HBS was 65.00% and the positive rate of HBV-DNA was 68.33%. The positive rate of HBsAg was statistically different (χ2 = 47.21, P <0.001). There was a linear positive correlation between L HBS level and HBV-DNA copy number (r = 0.965). Conclusion The detection of L-HBS and HBV-DNA is sensitive but not sensitive. L HBS detects the extent of virus replication in infected individuals, especially in HBeAg-negative patients.