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T_3的脱氨基代谢物三碘甲腺乙酸(Triac)在体内有弱的拟甲腺活动,但在体外它是~(125)Ⅰ—T_3与甲腺激素受体(RT)结合有力的竞争性抑制剂。为了探讨用~(125)Ⅰ—Triac测定RT,作者比较了大鼠肝核kcl提取液中~(125)Ⅰ-Triac和~(125)Ⅰ-T_3的结合特性。 大鼠经断头处死,取出肝脏并收集血液备用。所有步骤均在2—4℃冷室中进行。肝脏剪碎后制成匀浆,经悬浮及离心而洗涤。洗过的沉淀块悬浮于内含0.4M KCl的提取液中,离心得到的上清液
Triac, the deaminated metabolite of T_3, has weak gonadotropin activity in vivo, but in vitro it is a potent competitive inhibitor of 125I-T_3 binding to the thyroid hormone receptor (RT) Inhibitors. To investigate the determination of RT using ~ (125) I-Triac, the authors compared the binding properties of ~ (125) I-Triac and ~ (125) I-T_3 in rat liver nuclear kcl extract. Rats were sacrificed by decapitation, the liver was removed and the blood was collected for later use. All steps are carried out in a 2-4 ° C cold room. Liver homogenate after shearing, washing by suspension and centrifugation. The washed pellet is suspended in an extract containing 0.4 M KCl and the resulting supernatant is centrifuged