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目的 :研究白纹伊蚊在登革病毒传播中的作用。方法 :应用C6 /36细胞培养分离病毒结合RFPCR检测 ;用间接免疫荧光法 (IFA)检测血清抗体。结果 :饱吸感染发病乳鼠血的白纹伊蚊 ,对DENⅠ～Ⅳ型有较高的感染率 (50 %～ 58% ) ,通过叮咬敏感动物 (乳鼠 )也能传播这 4个型的DEN。分批检测感染雌蚊的子 1代卵、幼虫及雌、雄成虫 ,证实白纹伊蚊能经卵传递DEN ,至少可传 2代以上。结论 :该蚊对DEN有较大的媒介效能 ,且在维持DEN病毒的自然循环中起重要作用
Objective: To study the role of Aedes albopictus in the transmission of dengue virus. Methods: The virus was isolated by C6 / 36 cell culture and combined with RFPCR. The serum antibody was detected by indirect immunofluorescence assay (IFA). Results: The infection of Aedes albopictus in the blood of suckling rats with full infection was associated with a higher infection rate (50% -58%) to DEN Ⅰ ~ Ⅳ and was also transmitted by bite sensitive animals (suckling mice) DEN. Infected by the first batch of eggs of female mosquitoes, larvae and male and female adults, confirmed that Aedes albopictus can be transmitted by egg DEN, at least pass more than 2 generations. Conclusion: The mosquito has greater vector potency on DEN and plays an important role in maintaining the natural circulation of DEN virus