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核酸杂交技术成为检测巨细胞病毒(CMV)感染的敏感、特异的方法。我们采用CMV·DNA分子杂交的方法,对617名孕妇中IgM阳性婴儿进行检测,以监测CMV宫内感染对胎儿的影响,现报道如下。 材料和方法 一、资料来源 1989年元月~1989年6月武昌地区孕3~6个月孕妇617名,取静脉血分离血清、-30℃保存备用。对在我院分娩13名CMV-IgM阳性孕妇胎儿的脐血或24小时内足跟血无菌收集,分离
Nucleic acid hybridization has become a sensitive and specific method of detecting cytomegalovirus (CMV) infection. We use CMV · DNA molecular hybridization method, 617 pregnant women in IgM-positive infants to detect CMV intrauterine infection on the fetus, are reported below. Materials and Methods I. Data source January 1989 to June 1989 Wuchang pregnant 6 to 6 months 617 pregnant women take venous blood serum, stored at -30 ℃ for use. 13 cases of CMV-IgM positive pregnant women in our hospital delivery of fetal umbilical cord blood or heel blood within 24h sterile collection, separation