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目的:探讨磁性聚乳酸羟基乙酸氧化苦参碱纳米粒(M-PLGA-OM-NP)对小鼠肝纤维化的防治作用。方法:运用复乳法制备M-PLGA-OM-NP;透射电镜观察纳米粒形态。采用二甲基亚硝胺(DMN)诱导小鼠肝纤维化模型,观察M-PLGA-OM-NP干预后肝脏生化指标及病理组织改变。结果:M-PLGA-OM-NP外观呈规则球形,平均粒径为146.5nm,载药量为7.61%,包封率为44.8%。M-PLGA-OM-NP组(外加磁场组和非磁场组)的丙氨酸转氨酶(ALT)水平较模型组下降;光镜下肝脏的纤维化程度较模型组明显减轻,较单纯氧化苦参碱(OM)用药组也明显改善;肝星状细胞活化的标志物α-SMA的表达较模型组及单纯OM用药组明显减弱。结论:M-PLGA-OM-NP在外加磁场的作用下,可增强OM的对DMN诱导的肝纤维化的防治作用。
Objective: To investigate the preventive and therapeutic effects of magnetic poly (lactic-co-glycolic acid) oxymatrine nanoparticles (M-PLGA-OM-NP) on hepatic fibrosis in mice. Methods: M-PLGA-OM-NP was prepared by double emulsion method. The morphology of nanoparticles was observed by transmission electron microscope. The model of hepatic fibrosis induced by dimethylnitrosamine (DMN) was used to observe the changes of liver biochemical indexes and pathological changes after M-PLGA-OM-NP intervention. Results: The appearance of M-PLGA-OM-NP was spherical. The average particle diameter was 146.5nm. The drug loading was 7.61% and the entrapment efficiency was 44.8%. The level of alanine aminotransferase (ALT) in M-PLGA-OM-NP group (applied magnetic field and non-magnetic field group) was lower than that in model group. The degree of fibrosis in liver in M-PLGA-OM-NP group was lighter than that in model group Alkaline (OM) treatment group also significantly improved; hepatic stellate cell activation of the marker α-SMA expression was significantly reduced compared with model group and OM alone group. CONCLUSION: M-PLGA-OM-NP can enhance the preventive and therapeutic effects of OM on DMN-induced hepatic fibrosis under the action of applied magnetic field.