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将经直接消化的斑马鱼头肾细胞或经短暂培养的尾鳍细胞核移植到同种成熟具核卵子中 ,移核卵可发育到原肠晚期 ;将移核卵发育成的囊胚细胞核作供体进行第 2次核移植 ,受体卵可发育到尾芽期或肌肉感应期。流式细胞仪测定 DNA含量表明 ,受体卵发育形成的囊胚细胞 DNA含量与正常囊胚细胞 DNA含量相同 ,由此可知 ,移核卵中的雌原核没有参与移核胚胎的发育。
The directly digested zebrafish head kidney cells or the transient culture of the caudate fin cells nucleus transplanted into the same kind of mature eggs, the nuclear transfer of eggs can develop into the late gut; The second nuclear transfer, the recipient egg can develop to the tail bud or muscle induction period. The DNA content of flow cytometry showed that the DNA content of blastocyst cells formed by the recipient egg development was the same as that of normal blastocyst cells. Thus, the prokaryotic nuclei in the nuclear transferred eggs did not participate in the development of nuclear transfer embryos.