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建立了一种测定磷酸盐缓冲体系中大豆卵磷脂含量的方法。利用无水乙醇 -石油醚 ( 60～ 90℃ )萃取剂从磷酸盐缓冲溶液中分离出大豆卵磷脂 ,再用钼蓝法对大豆卵磷脂含量进行测定。该方法操作简便 ,定量准确 ,重现性好 ,用于胰岛素口腔喷剂中大豆卵磷脂含量测定 ,样品平均回收率为 99.75% ,RSD=3.93%。
A method to determine the content of soybean lecithin in phosphate buffer system was established. Soybean lecithin was separated from phosphate buffer solution by extraction with anhydrous ethanol - petroleum ether (60 ~ 90 ℃), and the content of soybean lecithin was determined by molybdenum blue method. The method was simple, accurate and reproducible. The method was applied to the determination of soybean lecithin in oral spray of insulin. The average recovery was 99.75% and the RSD was 3.93%.