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目的 探讨ｐ１６ 基因及其蛋白异常表达与肺腺癌病期及转移的关系。方法 肺癌手术标本采用免疫组化 Ａ Ｂ Ｃ法分析ｐ１６ 蛋白的表达，其结果与ｐ１６ 基因第２ 外显子多聚酶链反应单链构象多态性分析（ Ｐ Ｃ Ｒ Ｓ Ｓ Ｃ Ｐ）结果进行对比。胸水中肺腺癌细胞采用 Ｐ Ｃ Ｒ Ｓ Ｓ Ｃ Ｐ分析。结果 （１）７４ 例肺腺癌和７６ 例肺鳞癌中ｐ１６ 蛋白阳性表达率分别为 ４７．３％ 和４７．４％ ，二组间无显著差异（ Ｐ＞ ０．０５）。但肺腺癌ｐ１６ 蛋白阴性表达频率与病期早晚和淋巴结转移部位密切相关；（２）ｐ１６ 蛋白表达下调的主要原因为ｐ１６ 基因纯合性缺失，点突变少见；（３）胸水肺腺癌细胞中ｐ１６ 基因缺失的发生率（７１．４％ ）显著高于Ⅰ期原发病灶（２２．２％ ）。结论 ｐ１６ 基因异常表达是肺腺癌发生的晚期事件，其异常表达与病期及转移密切相关。
Objective To investigate the relationship between the abnormal expression of p16 gene and its protein and the stage and metastasis of lung adenocarcinoma. Methods The expression of p16 protein was analyzed by immunohistochemical A B C method in surgical specimens of lung cancer. The results were consistent with the result of polymerase chain reaction-single strand conformation polymorphism analysis (P C R S S C P) in the second exon of p16 gene. comparing. Lung adenocarcinoma cells in the pleural fluid were analyzed by P C R S S C P . Results (1) The positive rates of p16 protein in 74 cases of lung adenocarcinoma and 76 cases of squamous cell carcinoma of lung were 47.3% and 47.4%, respectively. There was no significant difference between the two groups (P> 0.05). However, the frequency of negative expression of p16 protein in lung adenocarcinoma is closely related to the early stage of disease and lymph node metastasis; (2) The main reason for the down-regulation of p16 protein is the loss of homozygosity of p16 gene and rare point mutations; (3) pleural adenocarcinoma cells The incidence of p16 gene deletions (71.4%) was significantly higher than that of stage I primary lesions (22.2%). Conclusion The abnormal expression of p16 gene is a late event of lung adenocarcinoma. The abnormal expression of p16 gene is closely related to the disease stage and metastasis.