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从河北省土壤中分离获得一株苏云金杆菌新分离株 HBF- 1 ,对其基本生物学特性和杀虫活性进行了研究。结果表明 ,该菌株对黄褐丽金龟幼虫具有特异高杀虫活性 ,对铜绿丽金龟幼虫也表现出较高的杀虫活性 ;但对棉铃虫和玉米螟等鳞翅目幼虫 ,以及鞘翅目叶甲科的柳叶甲无杀虫活性。该菌株不产生﹀-外毒素。其生长对氨基酸需求的测定结果表明 ,异亮氨酸可抑制缬氨酸和亮氨酸的生物合成代谢 ;在异亮氨酸的存在下 ,该菌株对缬氨酸和亮氨酸具有特殊需求
A new strain of Bacillus thuringiensis HBF-1 was isolated from soil in Hebei Province. The basic biological characteristics and insecticidal activity of HBF-1 were studied. The results showed that the strain showed high insecticidal activity against Larvae larvae and showed high insecticidal activity against larvae of Culex pipiens. However, larvae of Lepidoptera such as Helicoverpa armigera and Corn borer, Willow armor insecticidal activity. This strain does not produce ﹀-exotoxin. The results of the determination of amino acid requirements for its growth show that isoleucine inhibits the biosynthesis and metabolism of valine and leucine. In the presence of isoleucine, the strain has special needs for valine and leucine