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目的:观察健脾解毒方对幽门螺杆菌(H.pylori)感染C57BL/6小鼠诱发胃癌过程中的胃粘膜血管新生和PTEN(Phosphatase and tensin homolog deleted on chromosome ten)/PI3K/AKT信号通路的影响.方法:建立H.pylori标准株NCTC11637感染C57BL/6小鼠诱发胃癌的动物模型,将200只C57BL/6小鼠随机分成5组,即对照组、模型组、健脾解毒方低中高剂量组,每组40只.健脾解毒方低、中、高剂量每只小鼠分别灌服健脾解毒方250、500、1000 mg·kg-1·d-1.72周后,尿素酶实验检测小鼠胃粘膜H.pylori感染情况,组织病理学检查小鼠胃粘膜癌变率,免疫组化法检测H.pylori感染对小鼠胃粘膜微血管密度(Microvessel density,MVD)、血管内皮生长因子(vascular endothelial growth factor,VEGF)和PTEN/PI3K/AKT信号通路的影响.结果:72周后,对照组、模型组、健脾解毒方低中高剂量组H.pylori感染率分别为0%,100%,3%,0%,0%,中药组感染率明显下降;各组小鼠胃癌发生率分别为为0%、26.3%、13.2%、10%、7.5%,中药组较模型组发生率明显减低(P=0.020,P=0.023,P=0.007).模型组MVD和VEGF表达量较对照组明显升高(P=0.002,P<0.001),中药组可降低MVD和VEGF表达.模型组p-PTEN和p-AKT的表达较对照组明显升高(均P<0.001),中药组可不同程度的降低p-PTEN和p-AKT的表达.结论:H.pylori长期感染C57BL/6小鼠可诱发胃粘膜癌变,增加胃粘膜MVD,促进VEGF表达,抑制PTEN活性,从而激活PI3K/AKT信号通路.健脾解毒方可降低小鼠胃粘膜H.pylori感染率,降低癌变率,抑制MVD和VEGF表达,减少PTEN失活,从而阻断PI3K/AKT信号通路可能是其预防胃癌的重要靶点.“,”Objective:To reveal the effect of Jianpi Jiedu recipe (JPJDR) on angiogenesis and the PTEN (Phosphatase and tensin homolog deleted on chromosome ten)/PI3K/AKT signaling pathway in the course of H. pylori infection-induced carcinogenesis of gastric mucosa in C57BL/6 mice. Methods:Two-hundred C57BL/6 mice were randomly divided into five groups (control group, model group, JPJDR low-dose group, JPJDR medium-dose group, and JPJDR high-dose group), 40 in each group. A mouse model of gastric cancer, induced by H. pylori standard strain infection, was established. The mice of JPJDR low-dose, middle-dose, and high-dose groups were intragastrically administered 250, 500, and 1000 mg/kg JPJDR per day, respectively. After 72 weeks, the H. pylori infection in gastric mucosa of the mice was analyzed by rapid urease test; the pathological changes in the gastric mucosa of mice were assessed by histopathological examination, and micro-vessel density (MVD), vascular endothelial growth factor (VEGF), and PTEN/PI3K/AKT levels were determined. Results:The incidence of gastric cancer in each group (control group, model group, JPJDR low-dose, medium-dose, high-dose group) was 0%, 26.3%, 13.2%, 10%, and 7.5% respectively. The incidence of gastric cancer in the Chinese medicine group was significantly lower than that of the model group (P =0.020, P=0.023, P=0.007). The expression of MVD and VEGF in the model group was significantly higher than that in the control group (P=0.002, P<0.001), while the expression of MVD and VEGF decreased in the Chinese medicine group. The expression of p-PTEN and p-AKT in the model group was significantly higher than that in the control group (All P < 0.001), while Chinese medicine could reduce the expression of p-PTEN and p-AKT to varying extents. Conclusion:Long-term infection of C57BL/6 mice with H. pylori induces gastric carcinogenesis, by increasing gastric mucosal MVD, promoting the expression of VEGF, inhibiting the activity of PTEN, and activating the PI3K/AKT signaling pathway. JPJDR can reduce the infection rate of H. pylori in mouse gastric mucosa, inhibit the expression of MVD and VEGF, and reduce the inactivation of PTEN.