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从制备的蚕豆萎蔫病毒（ＢＢＷＶ）抗血清中提纯ＩｇＧ，并用过碘酸钠法进行辣根过氧化物酶（ＨＲＰ）标记，建立了ＤＡＳ－ＥＬＩＳＡ检测ＢＢＷＶ的方法。ＤＡＳ－ＥＬＩＳＡ检测时包被ＩｇＧ以２６．９μｇ／ｍｌ效果最好，ＨＲＰ－ＩｇＧ结合物以１８００～１１６００效果最佳。ＤＡＳ－ＥＬＩＳＡ检测ＢＢＷＶ病叶粗汁液的最大稀释度为１３２０，检测提纯病毒的最低浓度为０．７４４μｇ／ｍｌ。田间病样测定表明，ＢＢＷＶ在蚕豆、豌豆、豇豆、大豆、茄子和辣椒等作物上发生很普遍，在菜豆上也有零星发生，而在莴苣、芹菜、白菜、萝卜、花椰菜上未测到ＢＢＷＶ。ＤＡＳ－ＥＬＩＳＡ检测结果与生物学检测结果基本相符，符合率达９７．６％。
IgG was purified from antisera of BBWV and horseradish peroxidase (HRP) was labeled with sodium periodate, and the method of DAS-ELISA to detect BBWV was established. DAS-ELISA detection of IgG coated with the best 26.9μg / ml, HRP-IgG conjugates to 1800 ~ 11600 best. The maximum dilution of the leaf juice of BBWV disease was 1320 by DAS-ELISA and the lowest concentration of purified virus was 0.744μg / ml. The results showed that BBWV was common in crops such as fava beans, peas, cowpea, soybean, eggplant and peppers, but sporadic occurrences in kidney beans, while no BBWV was detected in lettuce, celery, cabbage, radish and cauliflower. DAS-ELISA test results and biological test results basically consistent with a rate of 97.6%.