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目的通过药理实验确定椭圆叶花锚利胆作用的有效部位,并对其进行成分分离与鉴定。方法采用胆管引流法试验椭圆叶花锚的利胆作用,并确定了有效部位。再利用硅胶柱色谱、ODS、葡聚糖凝胶等色谱手段分离,通过1H-NMR、13C-NMR等波谱技术确定化合物的结构。结果从椭圆叶花锚利胆有效部位分离得到了9个化合物,分别为2,3,5-trimethoxy-1-O-primevero-syloxyxanthone(1)、2,3,4,5-etramethoxy-1-O-primeverosyloxyxanthone(2)、l,8-dihydroxy-3,5-dimethoxanthone(3)、2,3,4,5,7-pentamethoxy-1-O-primeverosyloxyxanthone(4)、2,3,4,7-etramethoxy-1-O-primeverosyl-oxyxanthone(5)、木犀草苷(6)、木犀草素(7)、齐墩果酸(8)、熊果酸(9)。结论其中化合物3为首次从椭圆叶花锚中分得。
OBJECTIVE To determine the effective sites of the choleretic effect of Anthozoa by pharmacological experiments and to isolate and identify the components. Methods The gallbladder drainage method was used to test the choleretic effect of the oval leaf anchor and the effective site was determined. The residue was separated by silica gel column chromatography, ODS, Sephadex or the like, and the structure of the compound was confirmed by spectroscopic techniques such as 1H-NMR and 13C-NMR. Results Nine compounds were isolated from the active fractions of C. lucidum, which were 2,3,5-trimethoxy-1-O-primevero-syloxyxanthone (1), 2,3,4,5-etramethoxy-1-O- primeverosyloxyxanthone (2), l, 8-dihydroxy-3,5-dimethoxanthone (3), 2,3,4,5,7-pentamethoxy-1-O- primeverosyloxyxanthone (4), 2,3,4,7-etramethoxy -1-O-primeverosyl-oxyxanthone (5), luteolin (6), luteolin (7), oleanolic acid (8), ursolic acid (9). Conclusions Among them, Compound 3 is the first isolated from Echinacea.