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红景天甙(Salidroside)生源途径分子机制的解析是利用基因工程、代谢工程技术合成目标化合物的基础。糖基化是红景天甙生物合成的最后一步反应。在前期工作中,本课题组率先报道了与红景天甙生物合成相关的3个尿苷二磷酸葡萄糖基转移酶(UGTs)基因,在体外酶学性质研究的基础上,利用根癌农杆菌和发根农杆菌介导分别建立了相关转基因体系,鉴别了红景天甙生物合成最适UGT及植物和毛状根生物反应器系统合成红景天甙的效率差异;酪醇(Tyrosol)是红景天甙糖基化反应的甙元底物分子,其具体的代谢通路及其调控机制仍不明确。针对酪醇生物合成来源主要存在两种观点:一是酪醇可能来自于苯丙烷代谢途径产生的4-香豆酸,该途径起源于苯丙氨酸;二是生物碱代谢途径的中间产物酪胺可能是酪醇生物合成的前体,该途径则起源于酪氨酸。在后续工作中,否定了酪醇来源于苯丙烷代谢途径的可能性,进一步的工作证实酪氨酸脱羧酶(TyrDC)在酪醇生物合成的起始反应中担负着重要功能,酪醇作为一种苯乙烷类化合物衍生物,其生物合成来源于生物碱代谢途径。
The molecular mechanism of Salidroside biogenesis is the basis for the synthesis of target compounds by genetic engineering and metabolic engineering. Glycosylation is the final step in the biosynthesis of salidroside. In our previous work, our group first reported three uridine diphosphate glucosyltransferase (UGTs) genes related to the biosynthesis of salidroside. Based on the in vitro enzymatic properties, we used Agrobacterium tumefaciens And Agrobacterium rhizogenes respectively. The optimal biosynthesis of salidroside was identified and the efficiency of salidroside synthesis between plant and hairy root bioreactor system was identified. Tyrosol was Rhodiola glycosylation of aglycone substrate molecules, the specific metabolic pathways and its regulatory mechanism remains unclear. The main sources of biosynthesis of tyrosol exist in two ways: First, tyrosol may be derived from the phenylpropanoid metabolic pathway generated 4 - coumaric acid, the pathway originated in phenylalanine; the second is the alkaloid metabolic pathway intermediates cheese Amines may be precursors of tyrosol biosynthesis and this pathway is derived from tyrosine. In the follow-up work, the possibility of tyrosine derived from phenylpropanoid metabolic pathway was negated. Further work confirmed that tyrosine decarboxylase (TyrDC) plays an important role in the initial reaction of tyrosol biosynthesis. Tyrosol as a The benzene derivatives derivatives, the biosynthesis derived from the alkaloid metabolic pathway.