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目的 探讨体外不同培养条件对小鼠神经干细胞增殖及分化的影响。方法 分别用条件培养基及 10ml·L-1、2 0ml·L-1、30ml·L-1、4 0ml·L-1、5 0ml·L-1、10 0ml·L-1胎牛血清 +条件培养基对小鼠胚胎脑组织进行培养 ,用免疫组织化学方法进行鉴定。随后对不同培养条件下、不同生长时期神经干细胞生物学特性进行光镜观察。结果 10ml·L-1、2 0ml·L-1胎牛血清 +条件培养基组培养的神经干细胞比单独条件培养基组克隆球多而大 ;30ml·L-1、4 0ml·L-1、5 0ml·L-1胎牛血清 +条件培养基组既有部分贴壁细胞 ,也有大量的克隆球形成 ,克隆球有伪足样突起 ,克隆球间存在着广泛网络结构 ,且单一神经干细胞核分裂旺盛 ;10 0ml·L-1胎牛血清 +条件培养基组以贴壁细胞为主。结论 在体外不同的培养条件下神经干细胞及其神经球增殖及分化不同
Objective To investigate the effects of different culture conditions on the proliferation and differentiation of neural stem cells in mice. Methods The conditioned medium and 10ml · L-1,2 0ml · L-1, 30ml · L-1,4 0ml · L-1, 50ml · L-1, 10ml · L-1 fetal bovine serum + Conditioned medium was used to culture mouse embryonic brain tissue and identified by immunohistochemistry. Then under different culture conditions, different growth stages of neural stem cells biological characteristics of light microscopy. Results 10ml · L-1,2 0ml · L-1 fetal bovine serum + conditioned medium group of cultured neural stem cells than the conditioned medium group cloned more and larger; 30ml · L-1,4 0ml · L-1, There were some adherent cells in the 5 0ml · L-1 fetal bovine serum + conditioned medium group, a large number of spheres were also formed, the pseudopod-like protuberances of the cloned spheres, extensive network structure between the cloned spheres and single neural stem cell mitosis Strong; 10 0ml · L-1 fetal bovine serum + conditioned medium group adherent cells based. Conclusion The proliferation and differentiation of neural stem cells and their neurospheres are different under different culture conditions in vitro