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目的观察微囊藻毒素-LR(MCLR)暴露对小鼠肝组织DNA甲基转移酶(DNMTs)活性的影响。方法 80只昆明小鼠,雌雄各半,随机分为4组,每组20只,即对照组(生理盐水)及MCLR低、中、高剂量组(MCLR5、10、20μg/kg),1次/d经腹腔注射染毒,分别于染毒第11、21 d测定肝功能生化指标,观察肝脏组织形态学变化,检测DNMTs酶活性。结果染毒11 d时,与对照组比较,低剂量MCLR组小鼠肝脏谷丙转氨酶(ALT)、谷草转氨酶(AST)和碱性磷酸酶(ALP)无明显变化,高剂量组ALT、AST、ALP酶活性均升高,差异有统计学意义(P<0.05);染毒21 d时,与对照组比较,各剂量MCLR组小鼠肝脏ALT和AST水平均升高,差异有统计学意义(P<0.05);染毒11、21 d时,低、中、高MCLR组小鼠DNMTs酶活性分别为(89 135.7±14 334.7)、(92 645.8±15 962.3)、(70481.3±15 621.3)和(59 115.6±9 884.6)、(83 310.7±15 066.3)、(86 612.5±20 572.8)RFU/(h.mg),均高于对照组,差异有统计学意义(P<0.05)。结论 MCLR暴露可引起ALT、AST、ALP水平升高,肝组织DNMTs酶活性升高。
Objective To investigate the effect of microcystin-LR (MCLR) exposure on DNA methyltransferase (DNMTs) activity in mice liver. Methods Eighty Kunming mice were divided into 4 groups randomly (n = 20), ie control group (normal saline) and MCLR low, medium and high dose groups (MCLR 5, 10 and 20μg / kg) / d by intraperitoneal injection of exposure, were measured on the 11,21 d after exposure to biochemical markers of liver function, liver histological changes were observed to detect DNMTs enzyme activity. Results Compared with the control group, the levels of ALT, AST and ALP in liver of mice in low dose MCLR group did not change significantly at the 11th day of exposure. The levels of ALT, AST, (P <0.05). Compared with the control group, the levels of ALT and AST in the liver of mice in each dose of MCLR increased at the 21st day of exposure (P <0.05), the difference was statistically significant (P < P <0.05). DNMTs activities of low, middle and high MCLR mice were (89 135.7 ± 14 334.7), (92 645.8 ± 15 962.3), (70481.3 ± 15 621.3) and (59 115.6 ± 9 884.6), (83 310.7 ± 15 066.3) and (86 612.5 ± 20 572.8) RFU / (h.mg), respectively, which were significantly higher than those in the control group (P <0.05). Conclusion Exposure to MCLR can induce the elevation of ALT, AST, ALP and increase the activity of DNMTs in liver tissue.