Synergistic antitumor effects of in vivo production of human endostatin and tissue inhibitor of meta

来源 :中华医学杂志(英文版) | 被引量 : 0次 | 上传用户:Truth_Tiger
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
Background Tissue inhibitor of metalloproteinase (TIMP)-1 is a multifunctional protein. The aim of the study was to examine the feasibility of using a combination of adenovirus-mediated gene delivery of TIMP-1 plus endostatin and cell transplantation techniques to treat tumor growth and metastasis in mouse melanoma.Methods A enzyme-linked immunosorbent assay (ELISA) was used to detect the level of TIMP-1 and endostatin in vitro and in vivo. A tumor bearing mouse model and an experimental lung metastasis model in animal experiments were used to explore the therapeutic effect of in vivo production of human TIMP-1 and endostatin after the implantation of primary fibroblasts infected with the indicated adenovirus into tumor-bearing mice and a cytochemical method was used to observe histopathological changes of the tumor. An experimental lung metastasis model was established by injecting B16BL6 cells into the tail vein of mice and adenovirus-infected primary fibroblasts were subcutaneously implanted into the mice 24 hours later. Twenty-one days after tumor cell injection, mice were sacrificed to examine the effect on nodules visible as black forms on the surface of the lungs in B16BL6 cells.Results TIMP-1 and endostatin were secreted into the supatants of cultures of Ad-TIMP-1 and Ad-End-infected mouse primary fibroblasts. We also observed that implantation of fibroblasts infected with Ad-TIMP-1 alone, Ad-End alone, or Ad-TIMP-1 plus Ad-End resulted in detectable blood levels which may clearly inhibit the tumor growth and metastasis in a murine melanoma model.Conclusion These results suggest the high capacity of transfection for the delivery of TIMP-1 or endostatin gene constructs into primary fibroblasts, and demonstrate that the implantation of TIMP-1 and endostatin producing fibroblasts at a site in vivo where direct secretion of TIMP-1 and endostatin into the blood is possible represented a promising approach for the development of cancer therapy.
其他文献
新版《建筑设计防火规范》(GB 50016—2014)增加了设置自动灭火系统的要求,涵盖了高层建筑内的变电所、配电间、综合管廊电缆舱等。有效选用自动灭火系统对于建筑物内人员及
军队医院根本的职能是为部队做好卫勤保障工作,确保官兵身体健康,确保战斗力提高,这是我军医院的根本宗旨。多年来,我们始终坚持了这一宗旨,不断创新为部队服务思路,不断提高
Background The rocking and instability of a loaded complete denture (CD) during lateral excursion reduce the bearing area under the denture base, causing locali
目的评估临床上淋巴结阴性(clinically node-negative,cN0)的甲状腺乳头状癌(papillary thyroid carcinoma,PTC)患者术前的临床和超声特征与中央组淋巴结转移(central lymph node metastasis,CLNM)的关系。方法回顾性分析514例在本院经过手术病理证实为PTC患者的临床及超声图像特征,评估指标包括年龄、性别、肿瘤大小及
我们采用CZ-1型乳化剂及自行研制的ZY-3型乳化装置合成的乳化柴油、通过在中原油田的应用,各项指标达到了国家标准,烟度值降低了42.9%,排放的CO值降低了41.2%,节油率28%,经济效益显著。 We use CZ-1 emul
以位于上海北外滩地区的金光中心项目为研究实例展开论述,就大型商业综合体绿色节能设计问题进行探讨。通过对景观设计、交通便捷、幕墙设计、机电设计、电梯选型、无障碍设
上海闵行星河湾中学为预制装配式建筑。从学校建筑设计,预制构件节点连接,预制构件标准化、模数化,降低成本,构件生产芯片(RFID)管理等方面,阐述了该项目的设计与创新点,为后