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目的:观察补骨防疏汤对骨质疏松症大鼠骨组织骨保护素(OPG)的影响。方法:Wistar大鼠分为4组,采用维甲酸70 mg.kg-1.d-1ig连续2周,制备大鼠骨质疏松模型,观察灌服中、高剂量补骨防疏汤(40,20 g.kg-1)治疗4,8,12周后大鼠骨组织OPG的变化及骨小梁形态计量学变化。结果:维甲酸ig 2周后,模型对照组大鼠骨小梁明显稀疏,骨小梁面积为(22.52±3.15)%,骨组织OPG的面积积分吸光度(IA)为0.29±0.15,均显著低于正常组(P<0.01),说明造模成功。补骨防疏汤治疗后中、高剂量组大鼠骨小梁面积为(36.41±1.40)%,(37.58±1.40)%,显著高于模型对照组(P<0.01),骨组织OPG的IA为2.41±1.40,3.07±0.40,OPG阳性表达明显增多(P<0.01),骨组织病理变化明显改善。结论:补骨防疏汤能显著提高骨质疏松症大鼠OPG表达,改善骨质疏松的病理变化。
Objective: To observe the effect of Bu Gu Fang Shu Tang on osteoprotegerin (OPG) in osteoporosis rats. Methods: Wistar rats were divided into 4 groups. The rat model of osteoporosis was induced by retinoic acid 70 mg.kg-1.d-1ig for 2 weeks. 20 g.kg-1) for 4, 8, and 12 weeks after treatment. The bone mineral density (OPG) and trabecular bone morphometry were also measured. Results: After 2 weeks of retinoic acid, the trabecular area of trabecular bone was (22.52 ± 3.15)% in the model control group, and the area integral absorbance (OPA) of OPG was 0.29 ± 0.15 In the normal group (P <0.01), indicating successful modeling. Compared with the model control group (P <0.01), the bone trabecular area of the middle and high dose groups was (36.41 ± 1.40)% and (37.58 ± 1.40)%, (2.41 ± 1.40, 3.07 ± 0.40), the positive expression of OPG was significantly increased (P <0.01), and the pathological changes of bone tissue were improved obviously. Conclusion: Bugu Fangshuitang can significantly increase the OPG expression in osteoporosis rats and improve the pathological changes of osteoporosis.