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目的研究刺五加注射液对大鼠坐骨神经损伤的机制及可能机制。方法 SD大鼠随机分为正常组、模型组、治疗组1(20mg/kg·d)和治疗组2(40mg/kg·d)。大鼠左侧坐骨神经夹持法建立坐骨神经损伤模型,手术当天开始腹腔注射给药,连续6周。观察大鼠左足皮肤温度、坐骨神经功能指数(SFI)和坐骨神经传导速度(SNCV)及神经组织形态学的改变,免疫组织化学染色法及Western Blot观测大鼠坐骨神经生长因子的表达。结果治疗组大鼠皮肤温度、坐骨神经功能指数、坐骨神经传导速度及神经组织形态学改变均优于模型组,神经生长因子的表达水平明显高于模型组。结论刺五加注射液对损伤后大鼠坐骨神经有促进修复的作用,其机制可能与促进神经生长因子的表达有关。
Objective To study the mechanism and possible mechanism of acanthopanax senticosus injury on the sciatic nerve in rats. Methods SD rats were randomly divided into normal group, model group, treatment group 1 (20mg / kg · d) and treatment group 2 (40mg / kg · d). The model of sciatic nerve injury was established by the left sciatic nerve clamp in rats. The model was injected intraperitoneally on the day of surgery for 6 weeks. The left foot skin temperature, sciatic nerve function index (SFI) and sciatic nerve conduction velocity (SNCV) and the histological changes of the nerve tissue were observed. The expression of nerve growth factor in the sciatic nerve was observed by immunohistochemical staining and Western Blot. Results The skin temperature, sciatic nerve function index, sciatic nerve conduction velocity and nerve histomorphology in treatment group were better than those in model group. The expression of nerve growth factor was significantly higher in model group than in model group. Conclusion Acanthopanax senticosus injection can promote the repair of rat sciatic nerve after injury, and its mechanism may be related to the promotion of the expression of nerve growth factor.