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目的探讨中药复方制剂(安多霖)对微波辐射后大鼠海马线粒体呼吸链细胞色素C氧化酶(cytochromec oxidase,COX)变化的影响。方法 100只Wistar雄性大鼠随机分为3个对照组,即正常对照组、辐射预防对照组、辐射治疗对照组和2个安多霖组,即3 g/(kg.d)安多霖预防组及3 g/(kg.d)安多霖治疗组,每组20只。安多霖预防组大鼠于给药后第15天进行微波辐射;安多霖治疗组大鼠于辐射后当天开始连续给药14 d,均1次/d。采用30 mW/cm2微波辐射15 min,于辐射后6 h和14 d活杀动物取海马,通过比色法检测大鼠海马COX活性的变化;采用实时定量PCR和Western印迹检测大鼠海马COXⅠ、ⅣmRNA以及COXⅠ蛋白表达变化。结果辐射预防和治疗两对照组于停药后6 h COX活性、COXⅠ、ⅣmRNA,COXⅠ蛋白均降低(P<0.05或P<0.01);安多霖预防组较辐射预防对照组COX活性及基因表达有不同程度提高,与正常对照组无明显差异;安多霖治疗组与辐射治疗对照组相比明显提高(P<0.05),且基本恢复正常。结论给予3 g/(kg.d)安多霖对微波辐射致大鼠海马线粒体呼吸链COX表达降低有较明显改善作用,其治疗效果更为显著。
Objective To investigate the effects of traditional Chinese medicine (Andorin) on changes of mitochondrial respiratory chain cytochrome c oxidase (COX) in hippocampus of rats after microwave irradiation. Methods One hundred (100) Wistar male rats were randomly divided into three groups: normal control group, radiation prevention control group, radiation treatment control group and two Andolim group, ie, 3 g / (kg.d) Group and 3 g / (kg.d) Andolin treatment group, 20 rats in each group. Rats in the Andorra preventive group were subjected to microwave radiation on the 15th day after administration. Rats in the Andorhidene treatment group were dosed continuously for 14 days on the day after irradiation, once a day. The hippocampus of hippocampus were harvested at 30 mW / cm2 for 15 min. The hippocampus was harvested at 6 h and 14 d after irradiation. The changes of COX activity in the hippocampus were detected by colorimetric assay. Ⅳ mRNA and COX Ⅰ protein expression changes. Results The levels of COX activity, COX Ⅰ, Ⅳ mRNA and COX Ⅰ protein in both radiation prevention and treatment groups decreased 6 h after drug withdrawal (P <0.05 or P <0.01). Compared with radiation preventive and control groups, COX activity and gene expression There was no significant difference with the normal control group; Andolian treatment group was significantly higher than that of the radiation treatment control group (P <0.05), and returned to normal. Conclusions Administration of 3 g / (kg · d) of Andolin can significantly reduce the COX expression in hippocampus mitochondrial respiratory chain induced by microwave radiation, and the therapeutic effect is more significant.