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青枯雷尔氏菌(Ralstonia solanacearum)致病力分化严重。为了解福建省青枯雷尔雷氏菌的致病力类型及与无毒基因组成关系,本研究以弱化指数为指标,对福建省内不同地区和寄主的63个青枯雷尔氏菌进行致病力测定,结果表明,供试的青枯雷尔氏菌可分为3种致病力类型:强致病力、过渡型和无致病力;进一步对供试菌株进行无毒基因avr A、pop P1和pop P2的检测,3个无毒基因分布频率不同,avr A基因分布频率最高,达79.37%,pop P1基因的分布频率最低为46.03%;不同寄主的菌株无毒基因组成不同,番茄(Lycopersicum esculentum)和辣椒(Capsicum annuum)寄主以分布3种和2种无毒基因为主,茄子(Solanum melongena)寄主主要分布2种无毒基因,花生(Arachis hypogaea)寄主的菌株无毒基因分布较少,只分布1种avr A基因或没有检测到无毒基因;不同地域的菌株无毒基因组成不同,以番茄寄主为例,建瓯玉山镇的菌株分布3种和2种无毒基因为主,分别占45.45%和31.82%,宁德屏南菌株中3个无毒基因均等分布,福州北峰菌株中只分布avr A和pop P2基因,二者均等分布,福州营口菌株中pop P2基因分布频率最高,达60.00%;不同致病力的菌株无毒基因组成不同,无致病力菌株分布3种和2种无毒基因为主,过渡型菌株分布2种无毒基因为主,而强致病力菌株分布1种无毒基因为主;青枯雷尔氏菌的无毒基因组成与致病力相关性分析表明,3个无毒基因分布均与致病力呈正相关,其中pop P1基因与菌株致病力相关性最高,达显著水平,其皮尔逊相关系数(Pearson correlation coefficient,PCC)值为0.31。本研究为植物青枯病预警和防控提供重要信息。
Pathogenicity of Ralstonia solanacearum is severe. In order to understand the pathogenicity of Escherichia coli and its association with non-toxic gene composition in Fujian Province, we used weakness index as an indicator to test the genetic diversity of 63 R. solanacearum strains from different regions and hosts in Fujian Province The results showed that Ralstonia solanacearum could be divided into three types of virulence: virulence, transitional and nonpathogenicity. The virulence genes avr A, pop P1 and pop P2, the distribution frequencies of three avirulent genes were different. The frequency of avr A gene was highest (79.37%) and that of pop P1 gene was the lowest (46.03%). The non-toxic genes of different host strains were different Lycopersicum esculentum and Capsicum annuum were mainly distributed in 3 and 2 non-toxic genes. Hosts of solanum melongena mainly distributed 2 non-toxic genes, while those of host strains of Arachis hypogaea were non-toxic Gene distribution is less, only a distribution of avr A gene or no non-toxic gene was detected; different regions of non-toxic strains of different genes to tomato host, for example, Jianou Yushan town strains distribution and 2 kinds of non-toxic Genes dominated, accounting for 45.45% and 31.82% respectively, Ningde Pingnan Three avirulent genes were equally distributed in the strains. Only avr A and pop P2 genes were distributed in Fuzhou Beifeng strains, and the two were equally distributed. The distribution frequency of pop P2 gene in Yingkou was the highest (60.00%). The strains with different virulence There were three kinds of non-virulent strains and two non-virulence genes, two non-toxic genes were mainly distributed in transitional strains, and one non-toxic gene was dominant in virulent strains. The results showed that the distribution of three non-toxic genes was positively correlated with the pathogenicity, of which the pop P1 gene had the highest correlation with the virulence of the strain, reaching a significant level, The Pearson correlation coefficient (PCC) was 0.31. This study provides important information for early warning and control of plant bacterial wilt.