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谷胱甘肽是一种氧自由基的有效清除剂,以还原的形式大量地存在于细胞中。作者模拟临床心脏直视手术和心脏移植的过程,将切除的小白鼠心脏设计成心肌缺血/再灌注的实验模型,评价NAC能否提高心肌中的硫氢基储存量。方法共取鼠心50个。置于非再循环的灌注装置上(Nonrecirculating Langendor-ff)。在心脏灌注期间,用充氧的KrebsHen-seleit(K-H)碱性溶液作为灌注液从升主动脉注入;经左房入左室安置球囊,注入盐水使左室舒张末压达1.3kPa(10mmHg);用
Glutathione is a potent scavenger of oxygen free radicals found in large quantities in reduced form in cells. The authors simulated clinical open-heart surgery and heart transplantation. The excised mouse heart was designed as an experimental model of myocardial ischemia / reperfusion to evaluate whether NAC could increase the amount of sulfhydryl groups stored in the myocardium. A total of 50 rat heart. Place on Nonrecirculating Langendor-ff. During cardiopulmonary perfusion, oxygenated KrebsHen-seleit (KH) alkaline solution was injected from the ascending aorta into the left atrium. The balloon was inflated via the left atrium into the left ventricle and saline was infused to bring the left ventricular end diastolic pressure to 1.3 kPa (10 mmHg );use