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目的探讨PCR斑点杂交技术在检测肺炎支原体(MP)感染中的临床应用价值。方法收集疑似MP感染患儿的呼吸道标本70份,提取DNA后采用PCR技术将MP特异性DNA片段进行扩增;比较传统PCR法和PCR斑点杂交技术检测MP感染的效能。结果 32份临床标本经传统PCR法和PCR斑点杂交技术检测MP均为阳性,28份临床标本检测均为阴性;10份临床标本PCR斑点杂交技术检测为阳性,但传统PCR法检测为阴性,配对样本χ2检验显示两法检测MP的差异有统计学意义(P<0.05)。PCR斑点杂交技术检测MP的灵敏度为82.5%,特异度为95.6%,诊断符合率为81.4%;均高于传统PCR法的69.6%、80.6%和50.2%。结论与传统PCR法比较,PCR斑点杂交技术检测MP的灵敏度和特异度高。
Objective To investigate the clinical value of PCR dot blot hybridization in the detection of Mycoplasma pneumoniae (MP) infection. Methods Totally 70 respiratory samples were collected from children with suspected MP infection. PCR was used to amplify MP-specific DNA after DNA extraction. The efficacy of MP infection by traditional PCR and PCR dot blot hybridization was compared. Results All 32 samples were positive by conventional PCR and PCR dot blot hybridization and all 28 samples were negative. 10 samples were positive by PCR dot blot hybridization, but negative by conventional PCR and paired The χ2 test showed that there was a significant difference between the two methods in detecting MP (P <0.05). The sensitivity, specificity and accuracy of PCR dot blot hybridization were 82.5%, 95.6% and 81.4%, respectively. Both were higher than 69.6%, 80.6% and 50.2% of the conventional PCR. Conclusion Compared with the traditional PCR method, the sensitivity and specificity of PCR dot blot hybridization to detect MP are high.