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以５－氟脲嘧啶（５－ｆｌｕｏｒｏｕｒａｃｉｌ，５－Ｆｕ）腹腔注射治疗了１２只携有金属硫蛋白（Ｍｅｔａｌｏｔｈｉｏｎｅｉｎ，ＭＴ）启动子和增强子序列、ｂｃｒ－ａｂｌｃＤＮＡ（ｐ２１０）序列及ＳＶ４０剪切和Ｐｏｌｙ（Ａ）信号序列的转基因慢粒白血病（ｃｈｒｏｎｉｃｍｙｅｌｏｉｄｌｅｕｋｅｍｉ－ａ，ＣＭＬ）ＭＴ／ｐ２１０ｂｃｒ－ａｂｌ小鼠，于治疗的第５ｄ及第１０ｄ从不同脏器提取总ＲＮＡ、ＤＮＡ及蛋白质，分别进行ＰＣＲ分析、ＲＴ－ＰＣＲ检测被转移基因的表达水平及免疫沉淀法分析ｐ２１０ｂｃｒ－ａｂｌ转基因产物的激酶活性．结果表明，被转移基因在脾脏和骨髓中表达水平较高，胸腺和肾脏中呈低水平表达．５－Ｆｕ活体治疗１０ｄ后，被转移基因的表达水平及其表达产物的激酶活性均被明显抑制
Twelve metallothionein (MT) promoter and enhancer sequences, bcr-ablcDNA (p210) sequence, and SV40 cleavage were treated with 5-fluorouracil (5-Fu) intraperitoneally. Poly(A) signal sequence in transgenic chronic myeloid leukemi-a (CML) MT/p210bcr-abl mice. Total RNA, DNA, and protein were extracted from different organs on the 5th and 10th day of treatment. PCR analysis was performed. , RT-PCR detection of the expression level of the transferred gene and immunoprecipitation analysis of p210bcr-abl transgene product kinase activity. The results showed that the transgene was expressed in the spleen and bone marrow, and expressed in the thymus and kidney. After 10 days of treatment with 5-Fu in vivo, the expression level of the transferred gene and the kinase activity of the expressed product were significantly inhibited