用基因枪法分别将带有潮霉素磷酸转移酶标记基因的pCI-Os07G0622000、pCI-AK072466两种克隆载体导入玉米自交系501,对自交系501幼胚和愈伤组织两种受体进行转化。结果表明,501的幼胚和愈伤组织的转化情况不同,平均再生频率分别为10.25%和0.85%;PCR分子检测表明,Os07G0622000和AK072466基因已整合到玉米基因组中,501幼胚和愈伤组织两种受体最终分别获得15株和2株转基因植株,平均转化率分别为1.67%和0.19%。在自交系501的两种受体中,幼胚更适合作基因枪转化的受体材料。 Two cloning vectors of pCI-Os07G0622000 and pCI-AK072466 with hygromycin phosphotransferase marker gene were introduced into maize inbred line 501 by particle bombardment respectively. Two inbred embryos and two callus receptors Conversion. The results showed that the transformation rate of immature embryos and callus of 501 was different, and the average regeneration frequency was 10.25% and 0.85%, respectively. PCR analysis showed that Os07G0622000 and AK072466 genes were integrated into maize genome. The two receptors eventually obtained 15 and 2 transgenic plants, respectively, with average conversion rates of 1.67% and 0.19%, respectively. Of the two receptors in inbred 501, immature embryos are more suitable as acceptor materials for particle gun transformation.