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目的:研究白英水提取物对人肺腺癌SPC-A-1细胞株的凋亡诱导作用及对其Bcl-2表达的影响;探讨白英提取物诱导SPC-A-1细胞凋亡的可能机制。方法:选用人肺腺癌SPC-A-1细胞株为研究对象,在体外与白英提取物4种不同浓度共同培养24、48、72、96h,采用MTT法检测白英提取物对SPC-A-1细胞增殖活性的影响;琼脂糖凝胶电泳、流式细胞仪检测白英水提取物处理的SPC-A-1细胞株细胞凋亡;比较白英提取物处理前后SPC-A-1细胞凋亡相关基因Bcl-2表达的变化。结果:白英提取物抑制SPC-A-1细胞增殖活性;琼脂糖凝胶电泳可见凋亡梯带;SPC-A-1细胞有很低的自然凋亡率(1.37±0.52)%,50.0mg/ml白英提取物作用SPC-A-1细胞48h后,凋亡率为(33.25±4.39)%(P<0.05);50.0mg/ml白英提取物处理48h后的细胞凋亡相关基因Bcl-2表达率(24.41±2.96)%比未经处理的细胞(53.53±4.80)%表达率低(P<0.001)。结论:白英水提取物对人肺腺癌SPC-A-1细胞株有抑制增殖、诱导其凋亡作用,细胞凋亡的机制可能与细胞凋亡相关基因Bcl-2表达下调有关。
AIM: To investigate the apoptosis-inducing effect of Baili Extract on human lung adenocarcinoma cell line SPC-A-1 and its effect on Bcl-2 expression, and to explore the possible mechanism of Bailing extract inducing apoptosis of SPC-A-1 cell line . Methods: Human lung adenocarcinoma cell line SPC-A-1 was selected as the research object and cultured in vitro at different concentrations of C. albicans at different concentrations for 24, 48, 72, and 96 hours. MTT assay was used to determine the effect of SPC- A-1 cell proliferation; Apoptosis of SPC-A-1 cell line treated with Bailingshui extract was detected by agarose gel electrophoresis and flow cytometry. Compared the apoptosis of SPC-A-1 cells before and after treatment Changes of Bcl-2 gene expression. Results: The extract of Baiying could inhibit the proliferation of SPC-A-1 cells. The apoptotic ladder was observed by agarose gel electrophoresis. The apoptotic rate of SPC-A-1 cells was 1.37 ± 0.52% The apoptosis rate of SPC-A-1 cells was (33.25 ± 4.39)% (P <0.05) after treated with 50.0mg / ml Baiying extract for 48h and the apoptosis-related gene Bcl -2 expression rate (24.41 ± 2.96)% was lower than that of untreated cells (53.53 ± 4.80)% (P <0.001). CONCLUSION: Baishui water extract can inhibit proliferation and induce apoptosis of human lung adenocarcinoma SPC-A-1 cell line. The mechanism of apoptosis may be related to the down-regulation of Bcl-2 expression.