由于苯具有骨髓毒性,可引起白血病,并广泛存在于工作环境及室内空气中,因此,评价苯的接触十分重要。与空气中苯、尿酚、尿粘康酸及尿苯基巯基尿酸等指标相比,血苯是一项监测职业苯接触的特异指标。本研究的目的是建立血苯的测定方法,并以此为基本手段在志愿者中进行苯的动力学研究。血苯苯接触组由3个健康的非吸烟者组成,在动态暴露室中接触高浓度苯30 mg/m~3和低浓度苯5.2mg/m~34h。从接触苯前开始收集第一次血样后,每小时收集2次共3h,并在停止接触前采集最后一次。停止高浓度苯接触后,每隔10min采血一次,之后采血间隔加长,在24h内共采集16个血样。停止低浓度苯接触后,采集5个血样。血苯用顶空气相色谱法,配以光离子检测器进行分析,其结果为:连续接触30mg/m~3苯小时,停止接触1后,血苯平均值达238nmol/L,16h后,为 Because benzene has myelotoxicity, can cause leukemia, and widespread in the working environment and indoor air, therefore, the evaluation of benzene contact is very important. And benzene in the air, urinary phenol, urine muconic acid and urinary phenylmercapto-uric acid and other indicators, blood benzene is a specific indicator of occupational exposure to benzene. The purpose of this study was to establish a method for the determination of blood benzene and use it as a basic tool for the kinetic study of benzene in volunteers. The benzene benzene exposure group consisted of 3 healthy non-smokers exposed to high concentrations of benzene 30 mg / m3 and low concentrations of benzene 5.2 mg / m3 to 34 hours in the dynamic exposure chamber. After the first blood sample was collected from the pre-exposure to Benzene, it was collected 2 times a hour for a total of 3 h and the last acquisition was taken before stopping the contact. After discontinuing the exposure to high concentrations of benzene, the blood was taken every 10 minutes, then the blood collection interval was extended, and a total of 16 blood samples were collected within 24 hours. After stopping the low-concentration benzene contact, 5 blood samples were collected. Blood benzene by headspace gas chromatography, coupled with photo-ion detector for analysis, the results are: continuous exposure to 30mg / m ~ 3 benzene hours, stop the contact 1, blood benzene average 238nmol / L, after 16h