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2015年3月调查了海南省海口、琼海、万宁、儋州等市(县)的辣椒炭疽病的发生与病情,对采集的样本分离出的7株纯菌株进行了致病性测定及形态学观察,通过生长速率法及孢子萌发抑制法分别测定嘧菌酯对辣椒炭疽病病原菌菌丝和孢子的影响。结果表明,菌株DM4-2和DZ1-3为黑点炭疽菌(Colletotrichum capsici),菌株NP3-9、NP5-3、DZ2-7、HN5-11和HN2-6均为胶胞炭疽菌(C.gloeosporioides)。各菌株对嘧菌酯的敏感性显示,相对于菌丝,其病菌孢子对嘧菌酯的敏感性更高;相对于胶胞炭疽菌,黑点炭疽菌菌株DM4-2和DZ1-3对嘧菌酯更为敏感。
In March 2015, the occurrence and condition of pepper anthracnose in Haikou, Qionghai, Wanning, Zhuozhou and other cities in Hainan Province were investigated. The pathogenicity of the seven pure strains isolated from the collected samples were tested and analyzed. Morphological observation was conducted to determine the effects of azoxystrobin on the pathogen mycelia and spores of pepper anthracnose by growth rate and spore germination inhibition. The results showed that the strains DM4-2 and DZ1-3 were Colletotrichum capsici, while the strains NP3-9, NP5-3, DZ2-7, HN5-11 and HN2-6 were Colletotrichum gloeosporioides (C. gloeosporioides). The sensitivity of each strain to azoxystrobin showed that the spore of the strain was more susceptible to azoxystrobin than the mycelia. Compared with Colletotrichum gloeosporioides, the strains of black spot anthrax, DM4-2 and DZ1-3, Fungal esters more sensitive.