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目的:建立一种可以同时测定人参药性菌质中8种人参皂苷(人参皂苷Rg1,人参皂苷Re,人参皂苷Rf,人参皂苷Rh1,人参皂苷Rb1,人参皂苷Ro,人参皂苷Rb2,人参皂苷Rd)含量的HPLC方法。方法:采用高效液相法,Inertsil ODS-SP色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-0.4%磷酸水梯度洗脱,流速1 m L·min-1,检测波长203 nm,柱温30℃,测定人参药性菌质8种皂苷的含量。结果:8种单体皂苷在100 min内可良好分离,线性关系良好(R2>0.999 7),平均加样回收率分别为100.15%,101.54%,101.87%,100.88%,102.10%,102.84%,101.38%,101.23%,RSD分别为1.6%,2.3%,1.9%,2.1%,1.5%,0.8%,2.7%,1.6%。结论:该研究提供了一种能方便、简单、准确、重复性好的HPLC分析人参药性菌质中8种人参皂苷含量的方法,可为人参药性菌质成分转化研究的质量标准制定提供科学依据。
OBJECTIVE: To establish a method for the simultaneous determination of 8 ginsenosides (ginsenoside Rg1, ginsenoside Re, ginsenoside Rf, ginsenoside Rh1, ginsenoside Rb1, ginsenoside Ro, ginsenoside Rb2, ginsenoside Rd) in ginseng- HPLC method. Methods: Inertsil ODS-SP column (4.6 mm × 250 mm, 5 μm) was used as the mobile phase. The mobile phase was eluted with acetonitrile-0.4% phosphoric acid. The flow rate was 1 m L · min- The column temperature was 30 ℃, and the content of eight ginsenosides in ginseng-medicinal fungi was determined. Results: The eight monomeric saponins were well separated within 100 min with a good linear relationship (R2> 0.999 7). The average recoveries were 100.15%, 101.54%, 101.87%, 100.88%, 102.10% and 102.84%, respectively. 101.38%, 101.23%, RSD were 1.6%, 2.3%, 1.9%, 2.1%, 1.5%, 0.8%, 2.7%, 1.6% respectively. CONCLUSION: This study provides a convenient, simple, accurate and reproducible HPLC method for the determination of eight ginsenosides in ginseng-like prototrophic bacteria and provides a scientific basis for the quality standardization of ginseng-derived prototrophic constituents .