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’大矮蕉’MusaAAAcv.GrandeNaine的胚性细胞悬浮系(ECS)在M2液体培养基中预培养1周和2周后,分别将其接种在RD1或M3培养基上,于光照或黑暗条件下进行体胚的再生.再生体胚在接种后3周左右开始出现,经8周的培养后,在不同预培养时间、再生培养基种类及培养条件下,胚性细胞(团)质量增长了约5～18倍,从沉积细胞体积(SCV)为1mL的ECS获得的再生体胚数为0 71×105～3 07×105.植株的再生率及从SCV为1mL的ECS获得的再生植株数也因上述体胚再生条件的不同而异.
Embryogenic cell suspension (ECS) of ’Banana’ MusaAAAcv.GrandeNaine was preincubated for 1 week and 2 weeks in M2 liquid medium and inoculated separately on RD1 or M3 medium under light or dark conditions Somatic embryos were regenerated.The regenerated somatic embryos began to appear about 3 weeks after inoculation.After 8 weeks of culture, the quality of embryogenic cells increased by about 30% in different pre-culture time, regeneration medium and culture conditions The number of regenerated embryos obtained from ECS with a volume of sediment (SCV) of 1 mL was 0 71 × 105 ~ 3 × 107 × 105. The regeneration rate of plants and the number of regenerated plants obtained from ECS with SCV of 1 mL Due to the above conditions of somatic embryo regeneration vary.