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Objective: TO study the effect of Reduqing (RDQ) Injection on the lipopolysaccharide (LPS)induced tissue factor and cytokine production in whole blood. Methods: Heparinized human blood was incubated with LPS in the presence or absence of RDQ. The plasma concentrations of TNF-α, IL-1β, IL-6, and IL-8were measured by enzyme-linked immunosorbent assays (ELISA) and the monocyte tissue factor activity wasmeasured by a one-stage tissue factor induced plasma clotting time assay. Results: RDQ was found to diminishthe LPS-induced increase of TNF-α, IL-1β and IL-6 in plasma but did not completely abolish their production.In contrast to the effect on these cytokines, RDQ caused further increase of the plasma level of IL-8 and themonocyte TF activity in the presence of LPS. Conclusions: In the in vztro whole blood assay system used inthis study, the decrease of LPS-induced production of TNF-α, IL-1β, and IL-6 was similar to a previous in vivostudy on the effect of RDQ on the production of these cytokines in response to two-time LPS injection in rabbits, while the increase of IL-8 and TF production was contradictory to the previous in vivo study. Potentialreasons contributing to the differences are discussed.
Method: Heparinized human blood was incubated with LPS in the presence or absence of RDQ. The plasma concentrations of TNF. Objective: To study the effect of Reduqing (RDQ) Injection on the lipopolysaccharide (LPS) induced tissue factor and cytokine production in whole blood. -α, IL-1β, IL-6, and IL-8were measured by enzyme-linked immunosorbent assays (ELISA) and the monocyte tissue factor activity wasmeasured by a one-stage tissue factor induced plasma clotting time assay. Results: RDQ was found To diminished LPS-induced increase of TNF-α, IL-1β and IL-6 in plasma but did not completely abolish their production.In contrast to the effect on these cytokines, RDQ caused beneficial increase of the plasma level of IL-8 and Themonocyte TF activity in the presence of LPS. Conclusions: In the in vztro whole blood assay system used in this study, the decrease of LPS-induced production of TNF-α, IL-1β, and IL-6 was similar to a previous in vivostudy On the effect of RDQ on the production of th Ese cytokines in response to two-time LPS injection in rabbits, while the increase of IL-8 and TF production was contradictory to the previous in vivo study. Potentialreasons contributing to the differences are discussed.