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为了解福州动物园内不同源性大肠杆菌的耐药性及亲缘情况,为大肠杆菌病防治提供依据,笔者用微量肉汤法对8种抗菌药物进行药敏试验、PCR法检测intⅠ1、blaamp C、gyr A基因,并采用脉冲场凝胶电泳(PFGE)分型方法对分离自不同源性的大肠杆菌进行DNA指纹图谱分型,比较菌株之间的亲缘关系。结果显示,不同源性大肠杆菌耐药率为84.30%,其中复方新诺明(66.94%)、四环素(65.29%)和头孢唑林(48.76%)耐药率最高;多重耐药以2~4重耐药为主,占67.86%;intⅠ1基因检出率为24.79%,gyr A基因检出率为83.47%,blaamp C基因检出率为85.95%;PFGE分型结果显示,各菌株之间相似系数大部分在40%~75%之间,少数在70%~75%。结果表明,福州动物园内大肠杆菌整体耐药情况较严重,intⅠ1、blaamp C、gyr A基因与多重耐药具有相关性;动物源性、环境源性和人员性之间存在的多重耐药大肠杆菌之间未发现同一菌株的垂直克隆传播,没有流行病学相关性。
In order to understand the resistance of Fuzhou zoos from different origins of Escherichia coli and their relatives, provide basis for the prevention and control of E.coli, the author used micro-broth method to conduct antibiotic susceptibility test on 8 kinds of antibacterial drugs, PCR method detected intⅠ1, blaamp C, gyr A gene was amplified by polymerase chain reaction (PCR). Pulsed-field gel electrophoresis (PFGE) typing method was used to genotype DNA from Escherichia coli isolated from different sources to compare the genetic relationship among strains. The results showed that the resistance rate of Escherichia coli with different origins was 84.30%, of which the highest rates were cotrimoxazole (66.94%), tetracycline (65.29%) and cefazolin (48.76%); Heavy drug resistance accounted for 67.86%; intⅠ1 gene detection rate was 24.79%, gyr A gene detection rate was 83.47%, blaamp C gene detection rate was 85.95%; PFGE typing results showed that the strains were similar Most of the coefficients between 40% to 75%, a few in 70% to 75%. The results showed that the overall resistance of Escherichia coli was more serious in Fuzhou zoo, and the correlation between intⅠ1, blaamp C, gyr A gene and multi-drug resistance was found. The multi-drug resistant Escherichia coli isolated from animals, environment, No vertical clonal transmission of the same strain was found, with no epidemiological correlation.