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背景与目的：探讨电化学发光法(electrochemiluminescence ECL)在检测孕产妇乙肝病毒中的应用价值,从而正确指导乙肝孕产妇产后的喂养方式。方法：用ELISA法和ECL法平行测定孕产妇血清乙肝病毒血清标志物(HBV-M),并对ELISA法检测表面抗原为阴性而ECL法为低浓度的标本,用荧光定量PCR测定病毒DNA。结果：ECL法表面抗原的检出率(9．66%)明显高于ELISA法(6．54%),p＜0．05,ELISA法检测为阴性而ECL法为低浓度的标本,病毒DNA均为阳性。结论：ECL法测定HVV-M的敏感性比ELISA法高,可以更灵敏的检出低浓度的表面抗原,从而可以更好的指导孕产妇产后的喂养方式,但HBcAb阳性率过高。
BACKGROUND & AIM: To investigate the value of electrochemiluminescence ECL in the detection of hepatitis B virus (HBV) in pregnant women, so as to correctly guide the postpartum feeding mode of hepatitis B pregnant women. Methods: Serum HBV-M was detected by ELISA and ECL in parallel. The negative samples were detected by ELISA and the ECL was negative. The viral DNA was detected by fluorescence quantitative PCR. Results: The detection rate of ECL surface antigen (9.66%) was significantly higher than that of ELISA method (6.54%), p <0.05. ELISA method was negative and ECL method was low concentration. Are positive. CONCLUSION: The sensitivity of ECV assay to HVV-M is higher than that of ELISA, which can detect the low concentration of surface antigen more sensitively, which can better guide the postpartum feeding mode of pregnant women. However, the positive rate of HBcAb is too high.