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目的:研究3′-大豆苷元磺酸钠(DSS)对大鼠视网膜缺血再灌注损伤的影响。方法:用夹闭大鼠一侧颈总动脉的方法进行动物建模,制备大鼠视网膜缺血模型。随机分成3组:生理盐水组、不同剂量DSS治疗组(1、2mg/kg)。在夹闭大鼠一侧颈总动脉前分别舌下静脉注射相DSS或生理盐水。在夹闭大鼠一侧颈总动脉前、后1h及灌注后1h分别行眼底荧光血管造影(FFA)和视网膜电流图(ERG)检查。结果:与对照组相比,再灌注后1h与缺血前比较,DSS治疗组ERG振幅具有显著性差异。各组荧光血管造影(FFA)显示,DSS组视网膜血管痉挛明显恢复,静脉血管不规则扩张和迂曲;FFA显示DSS组灌注后视网膜动脉充盈时间明显缩短,且具有一定的剂量依赖性。结论:DSS能扩张视网膜血管,对视网膜缺血再灌注损伤有明显的保护作用。
Objective: To study the effect of 3’-daidzein sulfonate (DSS) on retinal ischemia-reperfusion injury in rats. Methods: Animal model was established by clipping the common carotid artery in rats to prepare rat retinal ischemia model. Randomly divided into 3 groups: saline group, different doses of DSS treatment group (1,2 mg / kg). Before occlusion of the common carotid artery in rats, sublingual venous injection of phase DSS or normal saline respectively. Fundus fluorescein angiography (FFA) and retinal electrogram (ERG) were performed at 1 h before and 1 h after clamping the common carotid artery in rats. Results: Compared with the control group, the amplitude of ERG in DSS group was significantly different at 1 hour after reperfusion compared with that before ischemia. Fluorescent angiography (FFA) in each group showed that retinal vasospasm of DSS group recovered obviously and blood vessels irregularly dilated and tortuous. FFA showed that the filling time of retinal artery in DSS group was significantly shortened and had a dose-dependent manner. Conclusion: DSS can dilate the retinal blood vessels and have a significant protective effect on retinal ischemia-reperfusion injury.