论文部分内容阅读
目的:建立同时测定甘肃产蒙古黄芪药材中黄芪皂苷Ⅰ、黄芪皂苷Ⅱ、黄芪皂苷Ⅲ、黄芪甲苷含量的方法。方法:采用超高效液相色谱法。色谱柱为Waters Acquity,流动相为乙腈-0.3%甲酸溶液(梯度洗脱);蒸发光散射检测器(ELSD)参数为漂移管温度70℃,喷雾器温度42℃,氮气体积流量2.07 L/min。结果:精密度、稳定性、重复性试验的RSD≤1.22%;黄芪皂苷Ⅰ、黄芪皂苷Ⅱ、黄芪皂苷Ⅲ、黄芪甲苷的加样回收率分别为100.32%、99.51%、100.57%、100.21%,RSD分别为0.44%、0.39%、1.19%、0.65%。结论:该方法简便、快速,结果准确、可靠,重复性良好,可为甘肃黄芪的质量控制提供依据。
Objective: To establish a method for the simultaneous determination of astragaloside Ⅰ, astragalus saponin Ⅱ, astragaloside Ⅲ and astragaloside Ⅳ in Astragalus membranaceus from Gansu Province. Methods: Using ultra performance liquid chromatography. The column was Waters Acquity and the mobile phase consisted of acetonitrile-0.3% formic acid (gradient elution). The parameters of ELSD were drift tube temperature 70 ℃, nebulizer temperature 42 ℃ and nitrogen flow volume 2.07 L / min. Results: The RSD of precision, stability and repeatability test were less than or equal to 1.22%. The recoveries of astragaloside Ⅰ, astragaloside Ⅱ, astragaloside Ⅲ and astragaloside were 100.32%, 99.51%, 100.57% and 100.21%, respectively. , RSD were 0.44%, 0.39%, 1.19%, 0.65% respectively. Conclusion: The method is simple, rapid, accurate, reliable and reproducible. It can provide the basis for the quality control of Radix Astragali.