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目的研究乌司他丁(UTI)与1,6二磷酸果糖(FDP)对脑缺血-再灌注损伤保护作用,探讨乌司他丁(UTI)联合FDP对脑缺血.再灌注损伤的保护机制。方法纯种白兔随机分为四组(n=6):对照组、UTI保护组、FDP保护组及UTI+FDP保护组。四管闭塞法制成兔全脑缺血-再灌注损伤模型。分别缺血再灌注即刻给予生理盐水、UTI(10μ/kg)、FDP(200 mg/kg)及UTI+ FDP。再灌注后30 min、3 h、6 h静脉血测丙二醛(MDA)和谷光甘肽过氧化物酶(GSH-Px)。结果MDA和GSH-Px值显示对照组与UTI、FDP、及UTI+FDP组比较各组P<0.05。UTI与UTI+FDP组比较,各点P值均小于0.05;FDP与UTI+FDP组比较,30 min P>0.05,3 h后P<0.01。结论UTI联合FDP对脑缺血-再灌注损伤保护作用与单独应用差异明显。表明UTI及FDP对脑缺血-再灌注损伤有保护作用,二者合用较单独应用效果更好。
Objective To investigate the protective effect of UTI and FDP on cerebral ischemia-reperfusion injury and to investigate the protective effect of UTI combined with FDP on cerebral ischemia and reperfusion injury mechanism. Methods Pure white rabbits were randomly divided into four groups (n = 6): control group, UTI protection group, FDP protection group and UTI + FDP protection group. Preparation of Rabbit Model of Global Cerebral Ischemia - Reperfusion Injury by Four - Tube Occlusion. Immediately after ischemia and reperfusion, saline, UTI (10μ / kg), FDP (200 mg / kg) and UTI + FDP were given respectively. Serum malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) were measured at 30 min, 3 h and 6 h after reperfusion. Results The levels of MDA and GSH-Px in the control group were significantly lower than those in the UTI, FDP and UTI + FDP groups (P <0.05). Compared with UTI + FDP group, the P values of all the points were less than 0.05 in UTI and UTI + FDP groups; P <0.01 compared with UTI + FDP group after 30 min P> 0.05 and 3 h. Conclusion The protective effect of UTI combined with FDP on cerebral ischemia-reperfusion injury is obviously different from the single application. The results showed that UTI and FDP had a protective effect on cerebral ischemia-reperfusion injury.