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目的探讨血红素加氧酶(HO-1)在姜黄素减轻脂多糖(LPS)所致急性肺损伤(ALI)中的作用。方法将大鼠随机分为5组:正常组、ALI模型、LPS+姜黄素组、LPS+姜黄素+氯血红素(Hm)组以及LPS+姜黄素+锌原卟啉(ZnPP)组。给药后进行肺组织的形态学观察,并测定丙二醛(MDA)的含量以及HO-1的活性;RT-PCR和Western blot检测HO-1 mRNA和蛋白的表达情况。结果用LPS成功复制出ALI模型,同时,肺组织中MDA含量、HO-1蛋白活性、HO-1 mRNA以及蛋白水平均高于阴性对照组(P均<0.05);姜黄素+LPS组、姜黄素+LPS+Hm组肺组织损伤程度、MDA含量低于LPS模型组,但HO-1活性、mRNA和蛋白水平高于相应LPS组(P均<0.05)。LPS+姜黄素+ZnPP组肺损伤程度、MDA含量高于LPS组,而HO-1活性、mRNA和蛋白水平低于LPS组(P<0.05)。结论姜黄素对ALI的保护作用可能通过增强HO-1活性、上调mRNA和蛋白表达水平而实现的。
Objective To investigate the effect of heme oxygenase (HO-1) on the alleviation of acute lung injury (ALI) induced by lipopolysaccharide (LPS) by curcumin. Methods The rats were randomly divided into 5 groups: normal group, ALI model, LPS + curcumin group, LPS + curcumin + hemin group and LPS + curcumin + zinc protoporphyrin group. The morphology of lung tissue was observed after administration, and the content of malondialdehyde (MDA) and the activity of HO-1 were determined. The expression of HO-1 mRNA and protein were detected by RT-PCR and Western blot. Results The ALI model was successfully replicated by LPS. Meanwhile, the content of MDA, the activity of HO-1 protein and the level of HO-1 mRNA and protein in lung tissue were higher than those in the negative control group (all P <0.05). Curcumin + LPS group, Compared with LPS group, the levels of HO-1 activity, mRNA and protein in the LPS + Hm group were significantly lower than those in the LPS group (P <0.05). LPS + curcumin + ZnPP group lung injury, MDA content higher than LPS group, while HO-1 activity, mRNA and protein levels were lower than LPS group (P <0.05). Conclusion The protective effect of curcumin on ALI may be achieved by increasing HO-1 activity and up-regulating mRNA and protein expression.