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本文研究了经口服途径染锰对大鼠子代神经行为和脑锥体外系发育的影响.选择健康成年SD大鼠,分三组即低剂量染锰组(2g/L)、高剂量染锰组(10g/L)和对照组.于大鼠妊娠0天开始染锰,染锰组分别只供给Mucl_2水溶液饮食,对照组则供应自来水,直至子代120天龄止.染锰期结束后,分别作神经行为检测[(1)Elevated plus Arms Mzze迷宫;(2)Morris Water Maze迷宫)];尾状核、伏隔核面积及胶质细胞纤维酸蛋白免疫反应(GFAP);黑质、中脑腹侧被盖区和尾状核的酪氨酸氢氧化酶免疫反应(TH)等结果的微机图像分析检测.如果如下:(1)染锰组仔鼠在Elevated plus Arms Maze迷宫测试中,从中央区至进入第一臂的潜伏期缩短,但进入开放臂的次数与对照组无差异,表明仔鼠神经焦虑性未受明显影响;在Morris Water Maze(水迷宫)的肌力测试中,各组到达平台的时间无差异,表明肌力未受影响;(2)高剂量组见状核和伏隔核的面积,CFAP反应强度及反应阳性产物平均相对密度均较对照组显著升高;(3)高剂量组黑质、中脑腹侧被盖区和尾状核的TH免疫反应强度和反应阳性产物平均相对密度均较对照组明显下降,表明脑
In this paper, we investigated the effects of manganese doping orally on the neurobehavioral and extrapyramidal development of rat offspring.Selecting healthy adult SD rats, the rats were divided into three groups: low-dose group (2g / L) (10g / L) and control group.Making manganese at 0 day of gestation in the rats, the group fed with MnCl2 only supplied Mucl2 diet, while the control group supplied tap water until 120 days of age.After the end of the manganese dosing period, (1) Elevated plus Arms Mzze maze; (2) Morris Water Maze maze); caudate nucleus, nucleus accumbens area and glial fibrillary acidic protein immunoreactivity (GFAP); substantia nigra, Brain ventral tegmental area and caudate nucleus tyrosine hydroxylase enzyme reaction (TH) and other results of the computer image analysis of detection.If the following: (1) manganese group pups in the Elevated plus Arms Maze maze test, The latency from the central region to the first arm shortened, but there was no difference between the number of open arms and the control group, indicating that the neuronal anxiety of the pups was not significantly affected. In the muscle test of Morris Water Maze There was no difference in the time between groups reaching the platform, indicating that muscle strength was not affected; (2) (3) The intensity of TH immunoreaction and the positive reaction products of immunoreactivity in the substantia nigra, midbrain ventral tegmental area and caudate nucleus in high dose group were higher than those in control group The average relative density was significantly lower than the control group, indicating that the brain