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目的探索重组人粒细胞集落刺激因子(rhG-CSF)对8.0Gy60Coγ射线照射小鼠长期存活率及远后效应的影响。方法 70只雄性C57BL/6小鼠,随机分为正常对照、照射对照及rhG-CSF大、中、小剂量治疗共5组,除正常对照组外,其他4组均接受8.0Gy60Coγ射线照射。治疗组小鼠于照射后0.5和24h两次分别皮下注射rhG-CSF1000、500和250μg/kg,随时记录各组动物存活率,照射后70、160和300d进行外周血细胞计数分析,300d时检测各组存活小鼠造血和免疫相关指标。结果照射对照组动物照后19d内全部死亡,平均存活时间(12.1±3.0)d;rhG-CSF1000μg/kg治疗组照射后30、100和300d存活率分别为86.7%、86.7%和80.0%,死亡小鼠平均存活时间较照射对照组明显延长(P<0.01)。照后300d时,rhG-CSF1000μg/kg治疗组的外周血红细胞和血小板计数均与正常对照组没有统计学差异,骨髓混合细胞集落形成单位数量显著低于正常对照组(P<0.01);rhG-CSF治疗组的CD4+/CD8+比值倒置且250μg/kg剂量组明显低于正常对照组(P<0.05);rhG-CSF治疗组造血和免疫器官的组织病理切片结果显示仍然存在不同程度的病变。结论 rhG-CSF1000μg/kg治疗可以显著提高8.0Gy60Coγ射线照射小鼠存活率,但照射后300d造血和免疫系统相关指标尚未完全恢复正常。
Objective To explore the effect of recombinant human granulocyte colony-stimulating factor (rhG-CSF) on long-term survival and long-term effects of 8.0Gy60Coγ-ray irradiated mice. Methods A total of 70 male C57BL / 6 mice were randomly divided into normal control group, irradiation control group and rhG-CSF treatment groups. A total of 5 groups were treated with 8.0Gy60Coγ-ray irradiation except for the normal control group. The mice in the treatment group were injected subcutaneously with rhG-CSF 1000, 500 and 250 μg / kg twice at 0.5 and 24 h after irradiation respectively. The survival rate of each group was recorded at any time. Peripheral blood cell counts were analyzed at 70, 160 and 300 d after irradiation. Group survival mice hematopoietic and immune related indicators. Results All animals died within 19 days after irradiation and the mean survival time was 12.1 ± 3.0 days. The survival rates at 30, 100 and 300 days after irradiation were 86.7%, 86.7% and 80.0%, respectively The average survival time of mice was significantly longer than that of the irradiated control group (P <0.01). The number of erythrocytes and platelets in the rhG-CSF 1000μg / kg treatment group was not significantly different from that in the normal control group at 300 days after irradiation. The number of colony forming units in the bone marrow mixed group was significantly lower than that in the normal control group (P <0.01) The ratio of CD4 + / CD8 + in CSF was upside down and the dose of 250μg / kg was significantly lower than that in normal control group (P <0.05). The histopathological sections of hematopoietic and immune organs in rhG-CSF group showed that there were still some lesions. Conclusion The treatment with 1000μg / kg rhG-CSF can significantly improve the survival rate of mice irradiated with 8.0Gy60Coγ ray, but the index of hematopoietic and immune system has not returned to normal completely 300d after irradiation.