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目的观察低频强声作用后大鼠海马回细胞形态、分布及凋亡的变化,探讨其可能机制。方法 SD大鼠随机分为:对照组8只和实验组40只。实验组:100Hz,140d B低频强声,1h/d,连续作用20d,在1d、10d、20d及停止作用后10d、20d处理大鼠,每次8只。采用HE染色观察海马回细胞形态和分布,TUNEL法检测海马回细胞凋亡,免疫组化检测海马回NSE和GFAP的表达。结果 1作用10d可见海马回细胞排列稍疏松紊乱,作用20d细胞排列疏松紊乱明显,而停止作用后10d细胞疏松紊乱现象较作用20d有所改善,停止作用后20d未进一步改善。2随着低频强声作用时间延长,海马回TUNEL阳性细胞率呈升高趋势,停止低频强声作用后有所回落,但仍高于对照组。3海马回NSE阳性细胞数量随着低频强声作用时间延长呈减少趋势,停止低频强声作用后有所增加,但仍少于对照组,而GFAP阳性细胞数量无明显变化。结论长时间低频强声作用可导致海马回神经元数量显著减少,停止作用后神经元数量部分恢复。
Objective To observe the changes of morphology, distribution and apoptosis of hippocampal gyrus in rats after low-frequency loud-sounding and to explore its possible mechanism. Methods SD rats were randomly divided into control group (n = 8) and experimental group (n = 40). The experimental group: 100Hz, 140d B strong bass sound, 1h / d, continuous action 20d, at 1d, 10d, 20d and stop the role of rats 10d, 20d, each 8. HE staining was used to observe the morphology and distribution of hippocampal gyrus cells. The apoptosis of hippocampal gyrus cells was detected by TUNEL and the expressions of NSE and GFAP were detected by immunohistochemistry. Results 10 days after treatment, the hippocampal gyrus cells were slightly loosely arranged, and the loosely arranged cells were obvious on the 20th day. However, the phenomenon of osteoporosis at 10 days after stopping was improved compared with that on the 20th day, and did not improve after 20 days. 2 With the prolongation of the duration of low-frequency acoustics, the positive rate of TUNEL-positive cells in the hippocampus tended to increase, and then decreased after the low-frequency acoustics was stopped, but still higher than that of the control group. The number of NSE-positive cells in the hippocampus returned to a trend of decreasing with the prolongation of low-frequency acoustical stimulation, and increased after stopping the low-frequency acoustical stimulation, but still less than that of the control group. There was no significant change in the number of GFAP positive cells. Conclusion Long-term high-frequency low-frequency sound can significantly reduce the number of neurons in the hippocampus and partially restore the number of neurons after stopping the action.