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为研究催产素(OT)与应激过程的关系,本实验采用免疫组织化学(PAP)法,微机图像分析仪相对定量测定,观察了烫伤后即刻、1、2、3和4h大鼠下丘脑室旁核OT阳性细胞的变化。每只鼠取OT阳性细胞面积最大的8张切片之和作为相对总面积进行比较。结果显示对照鼠的室旁核OT阳性细胞的相对总面积为(1.51±0.13)×10~5μm~2;烫后2h鼠的相对总面积为(2.95±0.31)×10~5μm~2,比对照鼠增大了95％(P<0.01),提示OT参与应激过程。
In order to study the relationship between oxytocin (OT) and stress process, this experiment used immunohistochemistry (PAP) method, relative quantitative determination of computer image analyzer to observe the hypothalamus immediately after burn, 1, 2, 3 and 4h Changes in paraventricular nucleus OT-positive cells. The sum of the 8 slices with the largest area of OT positive cells was taken as a relative total area for each rat. The results showed that the relative total area of OT-positive cells in the paraventricular nucleus of control rats was (1.51±0.13)×10~5μm~2; the relative total area of rats at 2h after scalding was (2.95±0.31)×10~5μm~2. The control rats increased by 95% (P<0.01), suggesting that OT is involved in the stress process.